Cloning and Nucleotide Sequence of Carbaryl Hydrolase Gene (cahA) from Arthrobacter sp. RC100(ENVIRONMENTAL BIOTECHNOLOGY)
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概要
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A carbaryl hydrolase gene (cahA) encoded on the plasmid pRC1 in Arthrobacter sp. RC100 was cloned and sequenced. The entire region of the deduced amino acid sequence was found to be homologous to that of an amidase family. Parts of the consensus sequences of the amidase gene have been identified in CahA from strain RC100. CahA was overexpressed in Escherichia coli JM109, and the enzyme was purified to homogeneity by protamine sulfate treatment, ammonium sulfate precipitation, and hydrophobic and anion-exchange chromatographies. The purified enzyme showed hydrolase activity toward 1-naphthylacetamide and isobutyramide but showed no activity toward 1-naphthylacetate. This is the first report of an amidase that is able to hydrolyze N-methylcarbamate pesticides.
- 社団法人日本生物工学会の論文
- 2006-05-25
著者
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Ohnishi-Kameyama Mayumi
National Food Research Institute, Ministry of Agriculture, Forestry and Fishers
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Hashimoto Masayuki
Division of Radiology, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine
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Hashimoto Masayuki
Division Of Gene Research Department Of Life Science Research Center For Human And Environmental Sci
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Ohnishi-kameyama Mayumi
National Food Research Institute
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Ohnishi-kameyama Mayumi
National Research Institute Of Food
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Tago Kanako
United Graduate School Of Agricultural Science Gifu University
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Mizutani Atsushi
Faculty Of Agriculture Shizuoka University
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HAYATSU Masahito
Faculty of Agriculture, Shizuoka University
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Shimojo Takahiro
Faculty of Agriculture, Shizuoka University
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Hayatsu Masahito
Faculty Of Agriculture Shizuoka University
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Shimojo Takahiro
Faculty Of Agriculture Shizuoka University
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