Purification and Characterization of Fenitrothion Hydrolase from Burkholderia sp. NF100(ENVIRONMENTAL BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
The organophosphorus pesticide hydrolase was purified to homogeneity from Burkholderia sp. NF100 by detergent extraction of the cell membrane fraction, anion-exchange, chromatofocusing, and gel filtration chromatographies. The purified enzyme had a molecular mass of 55 kDa and a pI 5.8, and the hydrolase activity was strongly inhibited by EDTA, dithiothreitol (DTT), Hg^<2+> and 1, 10-phenanthroline. The optimum pH and temperature for the enzyme activity were 8.0 and 40℃, respectively. The enzyme hydrolyzed five organophosphorus pesticides.
- 社団法人日本生物工学会の論文
- 2006-01-25
著者
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Hashimoto Masayuki
Division of Radiology, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine
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Hashimoto Masayuki
Division Of Gene Research Department Of Life Science Research Center For Human And Environmental Sci
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Tago Kanako
United Graduate School Of Agricultural Science Gifu University
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HAYATSU Masahito
Faculty of Agriculture, Shizuoka University
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YONEZAWA SUMIKO
Faculty of Agriculture, Shizuoka University
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OHKOUCHI TOSHIHIDE
Faculty of Agriculture, Shizuoka University
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Hayatsu Masahito
Faculty Of Agriculture Shizuoka University
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Ohkouchi Toshihide
Faculty Of Agriculture Shizuoka University
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Yonezawa Sumiko
Faculty Of Agriculture Shizuoka University
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