A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100

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概要

• 論文の詳細を見る

• The fenitrothion hydrolase gene encoded on the plasmid pNF2 in Burkholderia sp. strain NF100 was cloned and sequenced. The 6.5-kbp EcoRI fragment contained three ORFs (ORF1 to 3) the deduced amino acid sequences of which shared no significant homology with any sequence present in databases. The results of frameshift mutation showed that ORF1 and ORF2 were essential for full enzymatic activity, and ORF3 was not involved in the degradation of fenitrothion. Fenitrothion hydrolase was located in the cell membrane fraction. The DNA region containing the fenitrothion hydrolase gene was spontaneously lost from pNF2.

• 日本微生物生態学会の論文

著者

• Hashimoto Masayuki

  Division of Radiology, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine

• Ninomiya Tomoyuki

  Faculty Of Agriculture Shizuoka University

• Hashimoto Masayuki

  Division Of Gene Research Department Of Life Science Research Center For Human And Environmental Sci

• Tago Kanako

  United Graduate School Of Agricultural Science Gifu University

• HAYATSU Masahito

  Faculty of Agriculture, Shizuoka University

• YONEZAWA SUMIKO

  Faculty of Agriculture, Shizuoka University

• OHKOUCHI TOSHIHIDE

  Faculty of Agriculture, Shizuoka University

• Hayatsu Masahito

  Faculty Of Agriculture Shizuoka University

• Ohkouchi Toshihide

  Faculty Of Agriculture Shizuoka University

• Yonezawa Sumiko

  Faculty Of Agriculture Shizuoka University

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