Development and Validation of a Microplate-based β-carotene Bleaching Assay and Comparison of Antioxidant Activity (AOA) in Several Crops Measured by β-carotene Bleaching, DPPH and ORAC Assays
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概要
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The reproducibility and reliability of the micro-β-carotene-linoleic acid bleaching (BCB) assay have been improved, enabling comparison to the antioxidant activity (AOA) of extracts from eleven kinds of crops evaluated by the oxygen radical absorbance capacity (ORAC) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. All assays were conducted using 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) as a calibrator, and AOAs were expressed as micromoles of Trolox equivalent per gram of weight. The coefficient variation (CV) of the BCB assay using a microplate was 14.40%, which was comparable to that of the ORAC assay (H-ORAC (14.10%), L-ORAC (18.76 %)).
- 2009-03-01
著者
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SHINMOTO Hiroshi
Tamagawa University
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Mikami Ichiho
National Food Research Institute
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Shinmoto Hiroshi
Tamagawa University School Of Agriculture
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YAMAGUCHI Minako
National Food Research Institute
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TSUSHIDA Tojirou
National Food Research Institute
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Mikami Ichiho
National Food Res. Inst. Tsukuba Jpn
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Shinmoto Hiroshi
Tamagawa Univ. Tokyo Jpn
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