Evaluation of Anti-inflammatory and Anti-allergic Effects of Food Components Using DNA Microarray Analysis
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概要
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Many people suffer from allergies, such as asthma, pollinosis and atopic dermatitis, as well as inflammatory diseases. To elucidate the anti-allergic and anti-inflammatory effects of food components, we developed a DNA microarray carrying genes related to immunity, inflammation and housekeeping. Results of gene expression analysis using the DNA microarray showed good reproducibility and correlated well with those of quantitative RT-PCR analysis. DNA microarray analysis showed that the butanol soluble fraction of bitter gourd placenta extract suppressed bacterial lipopolysaccharide (LPS)-induced inflammatory gene expression in mouse macrophage-like cells (RAW264.7). DNA microarray analysis demonstrated that the flavonoid fisetin suppressed the activated T cell membrane induction of gene expression in HMC-1 human mast cells. Thus, we demonstrated the anti-inflammatory effect of bitter gourd and anti-allergic effect of fisetin using DNA microarray analysis. Furthermore, DNA microarray analysis detected gene expression associated with skin irritation using a reconstructed human epidermal model.
- 独立行政法人 国際農林水産業研究センターの論文
著者
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Nagai Kanji
National Food Research Institute National Agriculture And Food Research Organization:mitsubishi Rayo
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OIKE Hideaki
National Food Research Institute, National Agriculture and Food Research Organization
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KOBORI Masuko
National Food Research Institute, National Agriculture and Food Research Organization
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Kobori Masuko
National Food Research Institute
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Oike Hideaki
National Food Research Institute (nfri) National Agriculture And Food Research Organization (naro)
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TAKAHASHI Yumiko
National Food Research Institute (NFRI), National Agriculture and Food Research Organization (NARO)
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TAKAHASHI Yumiko
National Food Research Institute, National Agriculture and Food Research Organization
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NAGAI Kanji
National Food Research Institute, National Agriculture and Food Research Organization
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