Evaluation of the Skin Irritation Using a DNA Microarray on a Reconstructed Human Epidermal Model(Molecular and Cell Biology)
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概要
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To avoid the need to use animals to test the skin irritancy potential of chemicals and cosmetics, it is important to establish an in vitro method based on the reconstructed human epidermal model. To evaluate skin irritancy efficiently and sensitively, we determined the gene expression induced by a topically-applied mild irritant sodium dodecyl sulfate (SDS) in a reconstructed human epidermal model LabCyte^<TM> EPI-MODEL (LabCyte) using a DNA microarray carrying genes that were related to inflammation, immunity, stress and housekeeping. The expression and secretion of IL-1α in reconstructed human epidermal culture is known to be induced by irritation. We detected the induction of IL-1α expression and its secretion into the cell culture medium by treatment with 0.075% SDS for 18h in LabCyte culture using DNA microarray, quantitative reverse-transcription polymerase chain reaction (RT-PCR) and ELISA. DNA microarray analysis indicated that the expression of 10 of the 205 genes carried on the DNA microarray was significantly induced in a LabCyte culture by 0.05% or 0.075% SDS irritation for 18h. RT-PCR analysis confirmed that SDS treatment significantly induced the expressions of interleukin-1 receptor antagonist (IL-1RN), FOS-like antigen 1 (FOSL1), heat shock 70kDa protein 1A (HSPA1) and myeloid differentiation primary response gene (88) (MYD88), as well as the known marker genes for irritation IL-1β and IL-8 in a LabCyte culture. Our results showed that a DNA microarray is a useful tool for efficiently evaluating mild skin irritation using a reconstructed human epidermal model.
- 公益社団法人日本薬学会の論文
- 2009-02-01
著者
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Nagai Kanji
National Food Research Institute National Agriculture And Food Research Organization:mitsubishi Rayo
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Niwa Makoto
Katakura Chikkarin Co. Ltd.
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OIKE Hideaki
National Food Research Institute, National Agriculture and Food Research Organization
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KOBORI Masuko
National Food Research Institute, National Agriculture and Food Research Organization
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Oike Hideaki
National Food Research Institute National Agriculture And Food Research Organization
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Kobori Masuko
National Food Research Institute
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Oike Hideaki
National Food Research Institute (nfri) National Agriculture And Food Research Organization (naro)
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- Evaluation of the Skin Irritation Using a DNA Microarray on a Reconstructed Human Epidermal Model(Molecular and Cell Biology)
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