Purification and Some Properties of Tea Leaf Amine Oxidase
スポンサーリンク
概要
- 論文の詳細を見る
Tea leaf amine oxidase was purified by salting out and various column chromatographies. The molecular weight was estimated to be 162, 000 daltons by gel filtration on a Sephadex G-200 column. This enzyme was determinated to be a dimer of which the molecular weight was found to be 81, 000 daltons by SDS gel electrophoresis. The optimum pH for ethylamine oxidation by this enzyme was 7.0. The enzyme was stable up to 60°C From the results of a inhibition study with chelating agents, the enzyme was found to contain copper in the cupric state. Carbonyl reagents such as semicarbazide, hydroxylamine and hydrazine strongly inhibited the activity. The enzyme oxidized alkylamines, ethanolamine and benzylamine rapidly, and also oxidized tyramine, histamine and other diamines slowly. The Km value for ethylamine was 8.8 × 10-5M. Polyamines, secondary amines and tertiary amines were not oxidized.
- 社団法人 日本農芸化学会の論文
著者
-
Takeo Tadakazu
National Research Institute Of Tea
-
Tsushida Tojiro
National Food Res. Inst. Tsukuba Jpn
関連論文
- Konjac Glucomannan Consumption May Enhance Equol Production in Mice
- Inhibitory Effects of Apple Polyphenols on Differentiation of 3T3-L1 Cells into Adipocytes
- Progressive Effects of Phloridzin on Melanogenesis in B16 Mouse Melanoma Cells
- Effect of Ginsenosides and Red Ginseng Water Extract on Tumor Necrosis Factor-α Production by Rat Peritoneal Macrophages
- Mioga (Zingiber mioga Rosc.) Extract Prevents 3T3-L1 Differentiation into Adipocytes and Obesity in Mice
- Effect of Flavonoids on the Differentiation of 3T3-L1 Adipocytes
- Flavonoids Inhibit Cell Growth and Induce Apoptosis in B16 Melanoma 4A5 Cells
- Preparation of a Bispecific Antibody to Ovomucoid and Horseradish Peroxidase by Chemical Recombination Method
- Phloretin-induced Apoptosis in B16 Melanoma 4A5 Cells and HL60 Human Leukemia Cells
- Structure-activity Relationships of Flavonoids and the Induction of Granulocyticor Monocytic-Differentiation in HL60 Human Myeloid Leukemia Cells
- Analysis of Rice Proteins of Transgenic Rice Resistant to Rice Stripe Virus
- Proanthocyanidins from Barley Bran Potentiate Retinoic Acid-induced Granulocytic and Sodium Butyrate-induced Monocytic Differentiation of HL60 Cells
- Competitive ELISA of Bovine Lactoferrin with Bispecific Monoclonal Antibodies
- Isolation and Identificaiton of (-)-Quinic Acid as an Unidentified Major Tea-component
- The importance of linoleic acid and linolenic acid as precursors of hexanal and trans-2-hexenal in black tea
- Assessment of the Mutagenicity of Extracts of TMV-Coat-Protein-Gene Induced Transgenic Tomato by the umu-Test
- Dihydrogenistein-Producing Bacterium TM-40 Isolated from Human Feces
- Changes in the Chemical Composition and Quality of Black Tea Due to Pluckung Standards(Food & Nutrition)
- Conversion of Glutamic Acid to γ-Aminobutyric Acid in Tea Leaves under Anaerobic Conditions(Biological Chemistry)
- Coumarin and Flavone Derivatives from Estragon and Thyme as Inhibitors of Chemical Mediator Release from RBL-2H3 Cells
- Eggplant Extract Inhibits Melanogenesis in B16 Melanoma Cells.
- An Enzyme Hydrolyzing L-Theanine in Tea Leaves
- Withering Effect on the Aroma Formation Found during Oolong Tea Manufacturing
- Effects of (-)-Epicatechin on Oxidation of Theaflavins by Polyphenol Oxidase from Tea Leaves
- Purification and Some Properties of Tea Leaf Amine Oxidase
- Effects of Nitrogenous Fertilizers on the Chemical Composition of CTC Black Tea