Separation of Model Melanoidin into Components with Copper Chelating Sepharose 6B Column Chromatography and Comparison of Chelating Activity(Food & Nutrition)
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概要
- 論文の詳細を見る
Nondialyzable model melanoidin prepared from glucose-glycine was separated into 6 components by pH gradient elution on a copper chelated Sepharose 6B column. The separated components were measured for copper chelating activity, UV-VIS spectrophotometry, and electrofocusing. The UV-VIS spectra of these components were found to be almost the same, but the melanoidin components with stronger affinity to the Cu column were found to be smaller in Kd. Their electrofocusing profiles were similar, and the electrofocused bands were categorized into four major groups with p1 2.5-4.0. Major chelating activity of the melanoidin components was due to a band of pI 2.7 on the electrofocusing.
- 社団法人日本農芸化学会の論文
- 1991-06-23
著者
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Murata M
Nara University Of Education
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Murata Michiyo
Nara University Of Education
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MURATA Masatsune
Department of Nutrition and Food Science, Ochanomizu University
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TERASAWA Naoko
Faculty of Education, Kanazawa University
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Homma S
Department Of Nutrition And Food Science Ochanomizu University
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Homma Seiichi
Department Of Nutrition & Food Science Ochanomizu University
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Terasawa N
Faculty Of Education Kanazawa University
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Terasawa Naoko
Department Of Dermatology Kyoto City Hospital
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Murata Masatsune
Department Of Nutrition & Food Science Ochanomizu University
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HOMMA Seiichi
Department of Bioscience, Tokyo University of Agriculture
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