Synthesis of Arabinose-Containing Cell Wall Precursors in Suspension-Cultured Tobacco Cells II. : Partial Purification and Some Physical Characterization of an Intracellular Precursor of Cell Wall Glycoproteins

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Arabinose-containing macromolecules accumulate in the Golgi apparatus prior to their secretion into the cell wall. These intracellular macromolecules of suspension-cultured tobacco cells were labelled with ^<14>C-arabinose, extracted and partially characterized. The major component is a glycoprotein, in which arabinose is linked to hydroxyproline residues as oligosaccharides, among them mainly as disaccharides. A pulse-chase experiment showed the major glycoprotein to be a precursor of cell-wall materials. The glycoprotein has a density of 1.65 g/cm^3 indicating a high content of carbohydrate (≳90%), of which arabinose, galactose and uronates are the major components. The glycoprotein is highly acidic (pI≲1.3), probably due to the presence ofuronate. The large stokes' radius, equivalent to a 5×10^5-dalton protein, and a small S value (6.5 S) indicate a swollen structure for the molecule. These data indicate a close similarity of the glycoprotein to an extracellular arabinogalactan protein secreted into the culture medium.
日本植物生理学会の論文