Comparison of mutation spectra induced by ethylating agents in DNA-alkyltransferase-deficient mutants of E. coli

概要

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The mutational spectra of ethylating agents, N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) and N-ethyl-N-nitrosourea (ENU) , were investigated using lacZ reversion assay in Escherichia coli ada and ogt mutants. ENNG and ENU induced all types of base substitutions with G : C→A : T transitions most predominant followed by A T→G : C transitions. In an ada background, induction of G : C→A : T and A : T→G : C transitions were not enhanced, while 4 types of transversions (G : C→T : A, A : T→C : G, G : C→C : G and A : T→T : A) were induced more strongly than in a wild-type back-ground. In an ogt background, however, the opposite effects were observed, i.e. ENNG and ENU induced considerable increase of G : C→A : T and A : T→G : C transitions while no difference in transversions was observed between ogt mutant and its parent strain.
日本環境変異原学会の論文
2001-12-22