Improvement of (R)-1,3-butanediol production by engineered Escherichia coli(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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(R)-1,3-Butanediol is a valuable chemical extensively used as a key intermediate for the synthesis of pharmaceuticals and several industrial compounds. Despite its high demand, the production has been restricted from multi-step chemical production, petrochemical substrate requirement and a non-existence natural synthesis pathway from renewable biomass. In this study, an artificial synthesis route was genetically engineered in Escherichia coli MG1655 lacI^q to produce 1,3-butanediol from glucose. The selection of heterologous genes from several organisms and activity level of their corresponding gene products were demonstrated to be the key element for the product formation. Improvement of fermentation aeration and the application of fed-batch system significantly enhanced 1,3-butanediol production. Under the optimized conditions, 1,3-butanediol was produced up to 9.05 g/l (100.4 mM) with 98.5 ± 02% enantiomeric excess (% ee) of (R)-1,3-butanediol. This is the highest yield of 1,3-butanediol produced from glucose with the highest optical purity by the recombinant strain reported thus far.
- 2013-05-00
著者
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Kato Junichi
Department Of Agricultural Biological Chemistry College Of Bioresource Sciences Nihon University
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Kataoka Naoya
Department Of Biological And Environmental Chemistry Tottori University
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Nakashimada Yutaka
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Tajima Takahisa
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Vangnai Alisa
Department Of Biochemistry Faculty Of Science Chulalongkorn University
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Nakashimada Yutaka
Department of Moleculor Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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Kataoka Naoya
Department of Moleculor Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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Tajima Takahisa
Department of Moleculor Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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Vangnai Alisa
Department of Biochemistry, Faculty of Science, Chulalongkorn University:Center of Excellence for Environmental and Hazardous Waste Management (EHWM), Chulalongkorn University
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