Enhanced 2, 3-Butanediol Production by Addition of Acetic Acid in Paenibacillus polymyxa
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概要
- 論文の詳細を見る
By the addition of 150 mM acetate into a batch culture at an initial pH of 6.8, the production of 2, 3-butanediol(BDL) by Paenibacillus polymyxa reached 248 mM, yielding 0.87 mol・mol^<-1> glucose, where the ratio of acetate consumed to glucose consumed(A/C ratio) was calculated as 0.35 mol acetate mol^<-1> glucose. Therefore, a fed-batch culture was carried out by feeding glucose and acetate at a ratio of 0.35 mol acetate mol^<-1> glucose. In the fed-batch culture performed at pH6.8, BDL production reached 637 mM, yielding 0.81 mol・mol^<-1> glucose, although the A/C ratio was only 0.18 mol acetate mol^<-1> glucose. By decreasing pH to 6.3 in the fed-batch culture, BDL production reached 566 mM, yielding 0.88 mol・mol^<-1> glucose and the A/C ratio was 0.32 mol acetate mol^<-1> glucose. The optical purity of BDL, which was expressed as enantiomeric excess, was retained at more than 98% of the (R, R)-stereoisomer at the end of culture, which was comparable to that without acetate addition.
- 社団法人日本生物工学会の論文
- 2000-12-25
著者
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Nishio N
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Nishio Naomichi
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Marwoto Bambang
Dept. Mol. Biotechnol Grad. Sch. Adv. Sci. Matt. Hiroshima Univ.
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KAKIZONO TOSHIHIDE
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Kakizono T
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Kakizono Toshihide
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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NAKAHIMADA YUTAKA
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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MARWOTO BAMBANG
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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KASHIWAMURA TAKASHI
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Nakashimada Y
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Nishio Naomichi
Department Of Fermentation Technology Faculty Of Engineering Hiroshima Univeisity
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Nakashimada Yutaka
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Kashiwamura Takashi
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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