Production of 5-Aminolevulinic Acid by Methanogens

概要

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The possibility of 5-aminolevulinic acid (ALA) accumulation in a culture medium was studied using Methanosarcina barkeri growing on a methanol minimal medium. To effect the accumulation of ALA in the culture medium, some ALA analogues were tested as inhibitors of ALA dehydratase. It was found that ALA was accumulated when either levulinic acid or 4,6-dioxoheptanoic acid (DOH) was added, with the latter being more effective, even at a much lower concentration. It was also found that in M. barkeri 2-oxoglutarate reacted as a precursor on the formation of ALA, the production of ALA increasing to 83μM from 37μM with the addition of 30mM of 2-oxoglutarate compared with the control experiment. The continous production of ALA from methanol in fixed-bed reactors of M. barkeri was tested, resulting in a miximum production rate of 400μmol/l/d compared with 200μmol/l/d by Methanobacterium thermoautotrophicum growing on a H_2 and CO_2 minimal medium.
公益社団法人日本生物工学会の論文
1989-08-25