Methanogenesis of Glusoce by Defined Thermophilic Coculture of Clostridium thermoaceticum and Methanosarcina sp.

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Methanogenesis of glucose by defined thermophilic coculture of Clostridium thermoaceticum 1745 and Methanosarcina sp. CHTI 55 was carried out to estabilish a symbiotic culture. In the coculture, glucose was first converted to acetate(inhibitory product)by C. thermoaceticum and the acetate was subsequently reduces to CH_4 and CO_2 by Methanosarcina sp., Suggesting that acetoclastic methanogenesis was the rate limiting step of the system. The inoculum ratio of Methanosarcina sp.(M)to C. thermoaceticum(C), M/C, was a key parameter capable of controlling acetate disappearance during culture, i.e., an M/C of 9.5 resulted in acetate apearance at the beginning of culture, although no acetate accumulation was observed by and M/C of 45.8 yielding 80 mM methane from 40 mM glucose within 4d culture. The growth kinetics of each monoculture were studied in order to conduct kinetic analysis of the cocultere system. The inhibition kinetics of glucose(substrate)and acetate(product)on the growth of C. thermoaceticum were analyzed by a second-order substrate inhibition model coupled with a non-competitive product inhibition model. The analysis revealed that the substrate saturation constant values were K_s=80.1 mM glucose; substrate inhibition constant, K_i=104.4 mM glucose; and inhibition constant, K_p=0.218 mM(undissociated acetic acid); exponent of inhibition, n=1.04; and μ_m was 0.19h^<-1>. Using a similar second-order substrate inhibition model, the values for Methanosarcina sp. were K_s=0.13 mM; K_i=0.62 mM(undissociated acetic acid); and the maximum specific rate of CH_4 production, q_m=17.31 ml CH_4/g cell/h.
公益社団法人日本生物工学会の論文
1990-12-25

Methanogenesis of Glusoce by Defined Thermophilic Coculture of Clostridium thermoaceticum and Methanosarcina sp.

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