Comparison of Two Vectors for Functional Expression of a Bacterial Cytochrome P450 Gene in Escherichia coli Using CYP153 Genes

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概要

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• Two vectors, pT7NScamAB and pRED, have been used for the functional expression of bacterial class I cytochrome P450 (P450) genes in Escherichia coli, which utilize putidaredoxin reductase (CamA) and putidaredoxin (CamB), and the reductase domain of a self-sufficient P450RhF respectively, for electron transfer from NAD(P)H to a P450 protein. We here compared the efficiency of bioconversion with the two vectors towards n-octane, cyclohexane, n-butylbenzene, and 2-n-butylbenzofuran using two well-characterized CYP153A genes, aciA and CYP153A13a (P450balk). As for n-octane bioconversion, aciA and pT7camAB was the best combination for the production of 1-octanol and 1,8-octanediol. As for the bioconversion of cyclohexane, n-butylbenzene and 2-n-butylbenzofuran, CYP153A13a with pRED achieved the most efficient bioconversion, as compared by conversion ratio per active CYP153A protein content. It was also found that 2-n-butylbenzofuran is biotransformed into 4-benzofuran-2-yl-butyric acid via 4-benzofuran-2-yl-butan-1-ol with E. coli cells expressing CYP153A.

• 社団法人 日本農芸化学会の論文
• 2009-08-23

著者

• Fujita Naoya

  Marine Biotechnology Institute

• Sumisa Futoshi

  Marine Biotechnology Institute

• KABUMOTO Hiroki

  Bioresource Laboratories, Mercian Corporation

• IKENAGA Hiroshi

  Marine Biotechnology Institute

• Misawa Norihiko

  Marine Biotechnology Institute:(present Office)central Laboratories For Frontier Technology Kirin Ho

• Misawa Norihiko

  Marine Biotechnology Institute

• Shindo Kazutoshi

  Department Of Food And Nutrition Japan Women's University

• Arisawa Akira

  Bioresource Laboratories Mercian Corp.

• Arisawa Akira

  Bioresource Laboratories Mercian Corporation

• Misawa N

  Marine Biotechnology Institute:(present Office)central Laboratories For Frontier Technology Kirin Ho

• Shindo K

  Department Of Food And Nutrition Japan Women's University

• Sumisa Futoshi

  Marine Biotechnology Inst.

• Shindo Kazutoshi

  Japan Women's University

• Kabumoto Hiroki

  Bioresource Laboratories Mercian Corp.

• Kabumoto Hiroki

  Bioresource Laboratories Mercian Corporation

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