Production of Hydroxlated Flavonoids with Cytochrome P450 BM3 Variant F87V and Their Antioxidative Activities
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概要
- 論文の詳細を見る
A variant of P450 BM3 with an F87V substitution [P450 BM3 (F87V)] is a substrate-promiscuous cytochrome P450 monooxygenase. We investigated the bioconversion of various flavonoids (favanones, chalcone, and isoflavone) by using recombinant Escherichia coli cells, which expressed the gene coding for P450 BM3 (F87V), to give their corresponding hydroxylated products. Potent antioxidative activities were observed in some of the products.
- 公益社団法人 日本農芸化学会の論文
著者
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AOKI Yoko
Department of Medical Genetics, Tohoku University School of Medicine
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Shindo Kazutoshi
Department Of Food And Nutrition Japan Women's University
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Misawa Norihiko
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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OTOMATSU Toshihiko
KNC Bio Research Center, KNC Laboratories Co., Ltd.
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KITAMURA Emi
Department of Food and Nutrition, Japan Women's University
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MAEDA Chiemi
Department of Food and Nutrition, Japan Women's University
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OTA Chihiro
Department of Food and Nutrition, Japan Women's University
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OTA Chihiro
Department of Food and Nutrition, Japan Women's University
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KITAMURA Emi
Department of Food and Nutrition, Japan Women's University
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MISAWA Norihiko
Reseach Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
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AOKI Yoko
Department of Food and Nutrition, Japan Women's University
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