Increase in Pladienolide D Production Rate Using a Streptomyces Strain Overexpressing a Cytochrome P450 Gene(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
Pladienolide B and its 16-hydroxylated derivative(pladienolide D) are novel 12-membered macrolides produced by Streptomycesplatemis Mer-11107 showing strong in vitro and in vivo anti-tumor activity. While pladienolide B is mainly produced by this strain, pladienolide D is produced to a lesser extent. To facilitate the production of pladienolide D by biotransformation, we found that Streptomyces bungoensis A-1544 was able to hydroxylate pladienolide B at 16-position. We identified psmA from S. bungoensis A-1544, which encoded a pladienolide B 16-hydroxylase PsmA belonging to the CYP105 family of cytochrome P450. To increase the efficiency of pladienolide D production, we constructed recombinant S. bungoensis A-1544 overexpressing psmA and per- formed biotransformation of pladienolide B to pladienolide D. This biotransformation achieved a production level 15-fold higher than that using the control strain S. bungoensis A-1544/pIJ702.
- 公益社団法人日本生物工学会の論文
- 2008-06-25
著者
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Arisawa Akira
Bioresource Laboratories Mercian Corp.
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Nakashima Takashi
Bioresource Laboratories Mercian Corp.
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ARITOKU Yasuhide
Bioresource Laboratories, Mercian Corp.
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Tsuchida Toshio
Bioresource Laboratories Mercian Corp.
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MACHIDA Kazuhiro
Bioresource Laboratories, Mercian Corp.
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Aritoku Yasuhide
Bioresource Laboratories Mercian Corp.
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Machida Kazuhiro
Bioresource Laboratories Mercian Corp.
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