Production of Human Metabolites of Cyclosporin A, AM1, AM4N and AM9, by Microbial Conversion(Microbial Physiology and Biotechnology)
スポンサーリンク
概要
- 論文の詳細を見る
In humans, cyclosporin A (CyA) is primarily metabolized to two hydroxylated (AM1 and AM9) and one N-demethylated (AM4N) derivative. To produce these derivatives, 1237 actinomycetes were screened for their ability to convert CyA. Among them, 89 strains (7.2%) produced these derivatives from CyA. Finally, Dactylosporangium variesporum IFO 14104, Actinoplanes sp. ATCC 53771 and Streptosporangium sp. AF935 were selected for the production of AM1, AM4N, and AM9, respectively. Composition of the production medium and the incubation period with CyA were important for obtaining a high yield of CyA derivatives. Large-scale microbial conversion of CyA (1.5g) using a 30-l jar fermentor yielded 288mg of AM1 (19.2%), 147mg of AM4N (9.8%) and 115mg of AM9 (7.7%).
- 公益社団法人日本生物工学会の論文
- 2005-04-25
著者
-
TSUCHIDA TOSHIO
Bioresource Laboratories, Mercian Corp.
-
Yamada Tomoya
Bioresource Laboratories Mercian Corp.
-
Agematu Hitosi
Bioresource Laboratories Mercian Corp.
-
Tsuchida Toshio
Bioresource Laboratories Mercian Corp.
-
OHTA KAZUO
Bioresource Laboratories, Mercian Corp.
-
KANEKO KATSURA
Bioresource Laboratories, Mercian Corp.
-
Kaneko Katsura
Bioresource Laboratories Mercian Corp.
-
Ohta Kazuo
Bioresource Laboratories Mercian Corp.
関連論文
- Convenient Total Synthesis of NM-3, an Antiangiogenesis Agent, and Its Optical Resolution
- Increase in the Rate of L-Pipecolic Acid Production Using lat-Expressing Escherichia coli by lysP and yeiE Amplification(Microbiology & Fermentation Technology)
- Hydroxylation of Testosterone by Bacterial Cytochromes P450 Using the Escherichia coli Expression System
- Hydroxylation of Oleanolic Acid to Queretaroic Acid by Cytochrome P450 from Nonomuraea recticatena
- Organization of the Biosynthetic Gene Cluster for the Polyketide Antitumor Macrolide, Pladienolide, in Streptomyces platensis Mer-11107
- Production of Human Metabolites of Cyclosporin A, AM1, AM4N and AM9, by Microbial Conversion(Microbial Physiology and Biotechnology)
- Increase in Pladienolide D Production Rate Using a Streptomyces Strain Overexpressing a Cytochrome P450 Gene(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- One-pot fermentation of pladienolide D by Streptomyces platensis expressing a heterologous cytochrome P450 gene(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Biotransformation of L-Lysine to L-Pipecolic Acid Catalyzed by L-Lysine 6-Aminotransferase and Pyrroline-5-carboxylate Reductase(Microbiology & Fermentation Technology)