Gene Cloning and Function Analysis of Replication Factor C from Thermococcus kodakaraensis KOD1(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Replication factor C (RFC) catalyzes the assembly of circular proliferating cell nuclear antigen (PCNA) clamps around primed DNA, enabling processive synthesis by DNA polymerase. The RFC-like genes, arranged in tandem in the Thermococcus kodakaraensis KOD1 genome, were cloned individually and coexpressed in Escherichia coli cells. T.kodakaraensis KOD1 RFC homologue (Tk-RFC) consists of the small subunit (Tk-RFCS : MW=37.2kDa) and the large subunit (Tk-RFCL : MW=57.2kDa). The DNA elongation rate of the family B DNA polymerase from T.kodakaraensis KOD1 (KOD DNA polymerase), which has the highest elongation rate in all thermostable DNA polymerases, was increased about 1.7 times, when T. kodakaraensis KOD1 PCNA (Tk-PCNA) and the Tk-RFC at the equal molar ratio of KOD DNA polymerase were reacted with primed DNA.
- 社団法人日本農芸化学会の論文
- 2003-11-23
著者
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Imanaka Tadayuki
Department Of Applied Biotechnology Faculty Of Engineering Osaka University
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Imanaka Tadayuki
Department Of Biotechnology Faculty Of Eigineering Osaka University
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Imanaka Tadayuki
Department Of Synthetic And Biological Chemistry Graduate School Of Engineering Kyoto University
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Imanaka T
Department Of Biotechnology Faculty Of Engineering Osaka University
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Imanaka Tadayuki
Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering.kyoto Univ
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Esaka M
Hiroshima Univ. Hiroshima
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Itakura M
Institute For Genome Research University Of Tokushima
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Imanaka T
Dep. Of Biotechnology Coll. Of Life Sciences Ritsumeikan Univ.
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Esaka Muneharu
Graduate School Of Biosphere Science Hiroshima University
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KITABAYASHI Masao
Tsuruga Institute of Biotechnology, Toyobo Co., Ltd.
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NISHIYA Yoshiaki
Bio-division, Toyobo Co., Ltd.
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ITAKURA Mitsuo
Institute for Genome Research, University of Tokushima
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Imanaka T
Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceuti
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Kitabayashi M
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.:graduate School Of Biosphere Science Hiroshima Un
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Itakura Mitsuo
Institute For Genome Research Tokushima University
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