Isolation of a New Mixotrophic Bacterium Which Can Fix CO_2 and Assimilate Aliphatic and Aromatic Hydrocarbons Anaerobically
スポンサーリンク
概要
- 論文の詳細を見る
We isolated a new type of Gram-negative mixotrophic bacterium, Pseudomonas sp. HD-1,which is a facultative anaerobe. Cells were grown on CO_2 as a sole carbon source in the presence of H_2. Anaerobic growth of the strain was enhanced by the addition of aliphatic as well as aromatic hycrocarbons. When cells were grown on n-alkane anaerobically, many oil vacuoles formed in the cells and about 40-50% of the dry cell weight was lipid and oil. The total increase in the hydrophilic components of the cells indicated the anaerobic assimilation of aliphatic hydrocarbon. Electron microscopic observation showed that the cell surface was highly wavy and that the membrane had a multi-layer structure irrespective of the presence of oil.
- 公益社団法人日本生物工学会の論文
- 1993-10-25
著者
-
Imanaka Tadayuki
Department Of Applied Biotechnology Faculty Of Engineering Osaka University
-
Morikawa Masaaki
Department Of Biotechnology Department Of Material & Life Science Graduate School Of Engineering
関連論文
- Bacterial Alginate Lyase : Enzymology, Genetics and Application
- Cellular Toxicity of Cadmium Ions and Their Detoxification by Heavy Metal-Specific Plant Peptides, Phytochelatins, Expressed in Mammalian Cells^1
- Utilization of Immobilized Archaeal Chaperonin for Enzyme Stabilization
- Enhanced Signal Transduction by a Directly Fused Protein of Interleukin-6 and Its Receptor
- Unique Nucleoid Structure during Cell Division of Thermococcus kodakaraensis KOD1
- Alteration of Product Specificity of Cyclodextrin Glucanotransferase from Thermococcus sp.B1001 by Site-Directed Mutagenesis
- Effect of Polyamines on Histone-Induced DNA Compaction of Hyperthermophilic Archaea
- Highly Branched Oligosaccharides Produced by the Transglycosylation Reaction of Neopullulanase
- Gene Cloning and Sequence Analysis of Cobyric Acid Synthase and Cobalamin (5'-phosphate) Synthase from Hyperthermophilic Archaeon Pyrococcus sp. KOD1
- Biophysical Analysis of Heat-Induced Structural Maturation of Glutamate Dehydrogenase from a Hyperthermophilic Archaeon (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Mutational Effects on O^6-Methylguanine-DNA Methyltransferase from Hyperthermophile : Contribution of Ion-Pair Network to Protein Thermostability
- Contribution of Protein-Surface Ion Pairs of a Hyperthermophilic Protein on Thermal and Thermodynamic Stability
- Genetic, Enzymatic, and Structural Analyses of Phenylalanyl-tRNA Synthetase from Thermococcus kodakaraensis KOD1
- Biophysical Effect of Amino Acids on the Prevention of Protein Aggregation
- 超好熱菌由来タンパク質のさまざまな変性条件下での構造安定性
- Acceptor Specificity of 4-α-Glucanotransferase from Pyrococcus kodakaraensis KOD1, and Synthesis of Cycloamylose
- Characterization of FtsZ Homolog from Hyperthermophilic Archaeon Pyrococcus kodakaraensis KOD1
- Characterization and Application to Hot Start PCR of Neutralizing Monoclonal Antibodies against KOD DNA Polymerase
- Gene Analysis and Enzymatic Properties of Thermostable β-Glycosidase from Pyrococcus kodakaraensis KODI
- Crystallographic Studies on a Family B DNA Polymerase from Hyperthermophilic Archaeon Pyrococcus kodakaraensis Strain KOD1^1
- Phylogenetic Analysis and Effect of Heat on Conformational Change of Ferredoxin from Hyperthermophilic Archaeon Pyrococcus sp.KOD1
- Cloning and Expression of the 4-α-Glucanotransferase Gene from the Hyperthermophilic Archaeon Pyrococcus sp. KOD1,and Characterization of the Enzyme
- Cloning and Expression of the α-Amylase Gene from the Hyperthermophilic Archaeon Pyrococcus sp. KOD1,and Characterization of the Enzyme
- Production of Bacterial Alginate-Specific Lyase by Recombinant Bacillus subtilis
- Crystallization and Preliminary X-Ray Diffraction Studies of Bacterial Alginate Lyase
- Gene Cloning and Characterization of Aldehyde Dehydrogenase from a Petroleum-Degrading Bacterium, Strain HD-1
- Characterization of Petroleum-Degrading Bacteria from Oil-Contaminated Sites in Vietnam
- Equilibrium and Kinetic Stability of a Hyperthermophilic Protein, O^6-Methylguanine-DNA Methyltransferase under Various Extreme Conditions
- Rapid Method for Detection and Detoxification of Heavy Metal Ions in Water Environments Using Phytochelatin
- A Simple Method for Determination of Substrate Specificity of Alginate Lyases
- Purification and Characterization of α-Glucan Phosphorylase from Bacillus stearothermophilus
- Production and Some Properties of a Dextrin with a Narrow Size Distribution by the Cyclization Reaction of Branching Enzyme
- Expression of Recombinant Antibody against Cancer-Specific Carbohydrate
- COMPLETE GENOME ANALYSIS AND TARGETED GENE DISRUPTION IN THE HYPERTHERMOPHILIC ARCHAEON, THERMOCOCCUS KODAKARAENSIS(Life under Extreme Environments, International Symposium on Origins of Life and Astrobiology)
- Gene Cloning and Characterization of Fructose-1,6-Bisphosphate Aldolase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1
- Display of Heterologous Gene Products on the Escherichia coli Cell Surface as Fusion Proteins with Flagellin
- Isolation and Characterization of Xanthobacter polyaromaticivorans sp. nov. 127W That Degrades Polycyclic and Heterocyclic Aromatic Compounds under Extremely Low Oxygen Conditions
- Characterization of a Small Metalloprotease from Streptomyces caespitosus with High Specificity to Aromatic Residues
- Production and Characterization of Biosurfactants from Bacillus licheniformis F2.2(Microbiology & Fermentation Technology)
- Mutational and Structural-Based Analyses of the Osmolyte Effect on Protein Stability
- Highly Thermostable Neutral Protease from Bacillus stearothemophilus
- Diversity in Size and Alkaliphily of Thermostable α-Amylase-Pullulanases (AapT) Produced by Recombinant Escherichia coli, Bacillus subtilis and the Wild-Type Bacillus sp.
- Rational Design for Stabilization and Optimum pH Shift of Serine Protease AprN
- Nucleotide Sequence and Possible Functional Mechanism of the Transcriptional Activator Gene (preL) for Neutral Protease from Lactobacillus sp.
- Nucleotide Sequence of the Neutral Protease Gene (nprL) from Lactobacillus sp. and Characterization of the Enzyme
- Purification and Characterization of a New Metal Protease Which Hydrolyzes the Cyclic Decapeptide, Gramicidin S
- Cloning and Nucleotide Sequence of the Regulatory Gene, degM, for Minor Serine Protease in Baciilus subtilis
- Role of the Pre-Pro-Region of Neutral Protease in Secretion in Bacillus subtilis
- Isolation and Characterization of Psychrotrophs from Subterranean Environments
- High-Level Production of Erythritol by Strain 618A-01 Isolated from Pollen
- Isolation of Bacterial Strans Colonizable in Mosquito Larval Guts as Novel Host Cells for Mosquito Control
- Importance of an N-Terminal Extension in Ribonuclease HII from Bacillus stearothermophilus for Substrate Binding
- Gene Cloning of an Alcohol Dehydrogenase from Thermophilic Alkane-Degrading Bacillus thermoleovorans B23
- Characterization of Ribonuclease HII from Escherichia coli Overproduced in a Soluble Form
- Identification of the Gene Encoding Esterase, a Homolog of Hormone-Sensitive Lipase, from an Oil-Degrading Bacterium, Strain HD-1
- Stabilities of Chimeras of Hyperthermophilic and Mesophilic Glycerol Kinases Constructed by DNA Shuffling
- Laser Irradiated Growth of Protein Crystal
- Gentisate 1,2-Dioxygenase from Xanthobacter polyaromaticivorans 127W
- Gene Cloning, Overproduction, and Characterization of Thermolabile Alkaline Phosphatase from a Psychrotrophic Bacterium
- Gene Cloning and Biochemical Characterizations of Thermostable Ribonuclease HIII from Bacillus stearothermophilus
- Cleavage of Various Peptides with Pitrilysin from Escherichia coli : Kinetic Analyses Using β-Endorphin and Its Derivatives
- The Concept of the α-Amylase Family : Structural Similarity and Common Catalytic Mechanism
- Cloning and Expression of the BamHI Restriction-Modification System in Bacillus subtilis
- Effect of Growth Temperature and Growth Phase on the Lipid Composition of the Archaeal Membrane from Thermococcus kodakaraensis
- Role of Repetitive Nine-Residue Sequence Motifs in Secretion, Enzymatic Activity, and Protein Conformation of a Family I.3 Lipase
- Identification of the Phr-dependent heat shock regulon in the hyperthermophilic archaeon, Thermococcus kodakaraensis
- Continuous Productin of Panose by Immobilized Neopullulanase
- Construction of Inducible Secretion Vectors and Their Application for the Secretion of Foreign Extracellular and Intracellular Proteins in Bacillus subtilis
- Gene Cloning and Characterization of Recombinant Ribose Phosphate Pyrophosphokinase from a Hyperthermophilic Archaeon
- 656 Cloning and Analysis of 16 S-rRNA gene and Transcriptional Factor TF IID gene from Hyperthermophilic Archaeum strain KOD 1
- Gene Cloning and Function Analysis of Replication Factor C from Thermococcus kodakaraensis KOD1(Biochemistry & Molecular Biology)
- Gene Cloning and Polymerase Chain Reaction with Proliferating Cell Nuclear Antigen from Thermococcus kodakaraensis KOD1(Biochemistry & Molecular Biology)
- Isolation of a New Surfactin Producer Bacillus pumilus A-1,and Cloning and Nucleotide Sequence of the Regulator Gene , psf-1
- Protein Engineering of Penicillinase as Affinity Ligands for Bioprocessing
- Isolation and Characterization of Long-Chain-Alkane Degrading Bacillus thermoleovorans from Deep Subterranean Petroleum Reservoirs
- Family B DNA polymerase from a hyperthermophilic archaeon Pyrobaculum calidifontis : Cloning, characterization and PCR application(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Production of Alkane and Alkene from CO_2 by a Petroleum-Degrading Bacterium
- Modeling of Cephalosporin C Production and Its Application to Fed-batch Culture
- Regulaton of Cephalosporin C Produciton by Endogeneous Methionine in Cephalosporium acremonium
- Morphological Differenctitaion in Relation to Cephalosporin C Synthesis by Cephalosporium acremonium
- Effect of Glucose and Methionine Consumption Rates on Cephalosporin C Production by Cephalosporium acremonium
- Isolation and Application of Extremophiles.
- Analysis of a Negative Regulator, SoxR, for the Arthrobacter Sarosine Oxidase Gene
- Cloning and Sequencing of the Sarcosine Oxidase Gene from Arthrobacter sp. TE1826
- Highly Conservative Sequence in the Carboxyl Terminus of Sarcosine Oxidase is Important for Substrate Binding
- Cloning and Nucleotide Sequence of the Urea Amidolyase Gene from Candida utilis
- Gene Cloning and Characterization of Thermostable Peptidyl Prolyl cis-trans Isomerase (PPIase) from Bacillus stearothermophilus SIC1
- Isolation of a New Mixotrophic Bacterium Which Can Fix CO_2 and Assimilate Aliphatic and Aromatic Hydrocarbons Anaerobically
- Biological Threshold Values of Procaryotic Gene Expression Which Is Controlled by the DNA Inverted Repeat Sequence and the mRNA Secondary Structure
- Effect of Amino Acid Substitutions at the Active Center on the Activity of Bacillus stearothermophilus Alcohol Dehydrogenase
- Tumor Suppressive Monoclonal Antibody Belonging to the V_H 7183 Family Directed to the Oncodevelopmental Carbohydrate Antigen on Human Hepatocellular Carcinoma
- Isolatino and Characterization of Oxygen Sensitive Mutants of Escherichia coli
- Binding ability of chitinase onto cellulose : an atomic force microscopy study
- Cloning and Nucleotide Sequence of the Gene Coding for a Subunit of the Respiratory NADH Dehydrogenase from Bacillus stearothermophilus
- Effect of Cultivation Temperatures on Thermophilic and Mesophilic Enzyme Gene Expression in Bacillus subtilis
- Characteristics of Thermostable Pullulanase from Bacillus stearothermophilus and the Nucleotide Sequence of the Gene
- A New Way of Stabilizing Recombinant Plasmids
- Adaptation Strategy of Thermophiles toward Hyperthermophily and Their Molecular Bases
- Molecular bases of thermophily in hyperthermophiles
- Cellular Toxicity of Cadmium Ions and Their Detoxification by Heavy Metal-Specific Plant Peptides, Phytochelatins, Expressed in Mammalian Cells.