Isolatino and Characterization of Oxygen Sensitive Mutants of Escherichia coli
スポンサーリンク
概要
- 論文の詳細を見る
Fifty-five oxygen sensitive mutants of Escherichia coli K-12 were isolated by chemical mutagenesis, two of which were manganese superoxide dismutase (Mn-SOD) defective mutants. These mutants (no. 34,no. 58) could grow anaerobically in minimal medium, but not under aerobic conditions. Mn-SOD of no. 58 was induced by adding paraquat or o-phenanthroline. However, the induction level was much lower than that of the wild-type strain without induction, inferring that strain no. 58 is a repressor-overproducing mutant. Strain no. 34 produced a mutant Mn-SOD enzyme (M') which migrated slowly in native PAGE. Protein M' was purified from strain no. 34 and the molecular weight was determined by gel filtration. Protein M' was found to be a dimer of identical subunits, as is the wild-type enzyme. The specific activity of M' is the same as that of the wild type. From these results, it was inferred that the mutant enzyme (M') may be more positively charged or less negatively charged than the wild type and that the productivity of M' was much lower than that of the wild-type enzyme. It was found that multicopies of the ilvD gene complemented the auxotrophic properties of mutant no. 34 under aerobic conditions. Since the gene product (α, β-dihydroxyisovalerate dehydratase) is very susceptible to inactivation by superoxide radicals, a large amount of the enzyme must be needed for strain no. 34 to grow aerobically. In contrast, such a complementary gene was not cloned in strain no. 58.
- 公益社団法人日本生物工学会の論文
- 1992-11-25
著者
-
Imanaka Tadayuki
Department Of Applied Biotechnology Faculty Of Engineering Osaka University
-
Matsumura Yoshinobu
Department Of Biotechnology Faculty Of Engineering Osaka University
-
Matsumura Yoshinobu
Department Of Biotechnology Faculty Of Engineering And High Technology Research Center Kansai Univer
関連論文
- Bacterial Alginate Lyase : Enzymology, Genetics and Application
- Cellular Toxicity of Cadmium Ions and Their Detoxification by Heavy Metal-Specific Plant Peptides, Phytochelatins, Expressed in Mammalian Cells^1
- Utilization of Immobilized Archaeal Chaperonin for Enzyme Stabilization
- Enhanced Signal Transduction by a Directly Fused Protein of Interleukin-6 and Its Receptor
- Unique Nucleoid Structure during Cell Division of Thermococcus kodakaraensis KOD1
- Alteration of Product Specificity of Cyclodextrin Glucanotransferase from Thermococcus sp.B1001 by Site-Directed Mutagenesis
- Effect of Polyamines on Histone-Induced DNA Compaction of Hyperthermophilic Archaea
- Highly Branched Oligosaccharides Produced by the Transglycosylation Reaction of Neopullulanase
- Gene Cloning and Sequence Analysis of Cobyric Acid Synthase and Cobalamin (5'-phosphate) Synthase from Hyperthermophilic Archaeon Pyrococcus sp. KOD1
- Biophysical Analysis of Heat-Induced Structural Maturation of Glutamate Dehydrogenase from a Hyperthermophilic Archaeon (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Mutational Effects on O^6-Methylguanine-DNA Methyltransferase from Hyperthermophile : Contribution of Ion-Pair Network to Protein Thermostability
- Contribution of Protein-Surface Ion Pairs of a Hyperthermophilic Protein on Thermal and Thermodynamic Stability
- Genetic, Enzymatic, and Structural Analyses of Phenylalanyl-tRNA Synthetase from Thermococcus kodakaraensis KOD1
- Biophysical Effect of Amino Acids on the Prevention of Protein Aggregation
- 超好熱菌由来タンパク質のさまざまな変性条件下での構造安定性
- Acceptor Specificity of 4-α-Glucanotransferase from Pyrococcus kodakaraensis KOD1, and Synthesis of Cycloamylose
- Characterization of FtsZ Homolog from Hyperthermophilic Archaeon Pyrococcus kodakaraensis KOD1
- Characterization and Application to Hot Start PCR of Neutralizing Monoclonal Antibodies against KOD DNA Polymerase
- Gene Analysis and Enzymatic Properties of Thermostable β-Glycosidase from Pyrococcus kodakaraensis KODI
- Crystallographic Studies on a Family B DNA Polymerase from Hyperthermophilic Archaeon Pyrococcus kodakaraensis Strain KOD1^1
- Phylogenetic Analysis and Effect of Heat on Conformational Change of Ferredoxin from Hyperthermophilic Archaeon Pyrococcus sp.KOD1
- Cloning and Expression of the 4-α-Glucanotransferase Gene from the Hyperthermophilic Archaeon Pyrococcus sp. KOD1,and Characterization of the Enzyme
- Cloning and Expression of the α-Amylase Gene from the Hyperthermophilic Archaeon Pyrococcus sp. KOD1,and Characterization of the Enzyme
- Onset of Ischemic Colitis Following Use of Electrical Muscle Stimulation (EMS) Exercise Equipment
- Nicotine Suppresses Tunicamycin-Induced, But Not Thapsigargin-Induced, Expression of GRP78 during ER Stress-Mediated Apoptosis in PC12 Cells
- Production of Bacterial Alginate-Specific Lyase by Recombinant Bacillus subtilis
- Crystallization and Preliminary X-Ray Diffraction Studies of Bacterial Alginate Lyase
- Gene Cloning and Characterization of Aldehyde Dehydrogenase from a Petroleum-Degrading Bacterium, Strain HD-1
- Characterization of Petroleum-Degrading Bacteria from Oil-Contaminated Sites in Vietnam
- Equilibrium and Kinetic Stability of a Hyperthermophilic Protein, O^6-Methylguanine-DNA Methyltransferase under Various Extreme Conditions
- Rapid Method for Detection and Detoxification of Heavy Metal Ions in Water Environments Using Phytochelatin
- A Simple Method for Determination of Substrate Specificity of Alginate Lyases
- Purification and Characterization of α-Glucan Phosphorylase from Bacillus stearothermophilus
- Production and Some Properties of a Dextrin with a Narrow Size Distribution by the Cyclization Reaction of Branching Enzyme
- Expression of Recombinant Antibody against Cancer-Specific Carbohydrate
- COMPLETE GENOME ANALYSIS AND TARGETED GENE DISRUPTION IN THE HYPERTHERMOPHILIC ARCHAEON, THERMOCOCCUS KODAKARAENSIS(Life under Extreme Environments, International Symposium on Origins of Life and Astrobiology)
- Gene Cloning and Characterization of Fructose-1,6-Bisphosphate Aldolase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1
- Isolation and Characterization of Novel Bisphenol : A-Degrading Bacteria from Soils
- Display of Heterologous Gene Products on the Escherichia coli Cell Surface as Fusion Proteins with Flagellin
- Isolation and Characterization of Xanthobacter polyaromaticivorans sp. nov. 127W That Degrades Polycyclic and Heterocyclic Aromatic Compounds under Extremely Low Oxygen Conditions
- Characterization of a Small Metalloprotease from Streptomyces caespitosus with High Specificity to Aromatic Residues
- Production and Characterization of Biosurfactants from Bacillus licheniformis F2.2(Microbiology & Fermentation Technology)
- Mediastinal Pancreatic Pseudocyst Caused by Obstruction of the Pancreatic Duct Was Eliminated by Bromhexine Hydrochloride
- Highly Thermostable Neutral Protease from Bacillus stearothemophilus
- Diversity in Size and Alkaliphily of Thermostable α-Amylase-Pullulanases (AapT) Produced by Recombinant Escherichia coli, Bacillus subtilis and the Wild-Type Bacillus sp.
- Rational Design for Stabilization and Optimum pH Shift of Serine Protease AprN
- Nucleotide Sequence and Possible Functional Mechanism of the Transcriptional Activator Gene (preL) for Neutral Protease from Lactobacillus sp.
- Nucleotide Sequence of the Neutral Protease Gene (nprL) from Lactobacillus sp. and Characterization of the Enzyme
- Purification and Characterization of a New Metal Protease Which Hydrolyzes the Cyclic Decapeptide, Gramicidin S
- Cloning and Nucleotide Sequence of the Regulatory Gene, degM, for Minor Serine Protease in Baciilus subtilis
- Role of the Pre-Pro-Region of Neutral Protease in Secretion in Bacillus subtilis
- Isolation and Characterization of Psychrotrophs from Subterranean Environments
- High-Level Production of Erythritol by Strain 618A-01 Isolated from Pollen
- Isolation of Bacterial Strans Colonizable in Mosquito Larval Guts as Novel Host Cells for Mosquito Control
- Gene Cloning of an Alcohol Dehydrogenase from Thermophilic Alkane-Degrading Bacillus thermoleovorans B23
- Identification of the Gene Encoding Esterase, a Homolog of Hormone-Sensitive Lipase, from an Oil-Degrading Bacterium, Strain HD-1
- Gentisate 1,2-Dioxygenase from Xanthobacter polyaromaticivorans 127W
- The Concept of the α-Amylase Family : Structural Similarity and Common Catalytic Mechanism
- Cloning and Expression of the BamHI Restriction-Modification System in Bacillus subtilis
- Effect of Growth Temperature and Growth Phase on the Lipid Composition of the Archaeal Membrane from Thermococcus kodakaraensis
- Identification of the Phr-dependent heat shock regulon in the hyperthermophilic archaeon, Thermococcus kodakaraensis
- Continuous Productin of Panose by Immobilized Neopullulanase
- Construction of Inducible Secretion Vectors and Their Application for the Secretion of Foreign Extracellular and Intracellular Proteins in Bacillus subtilis
- Gene Cloning and Characterization of Recombinant Ribose Phosphate Pyrophosphokinase from a Hyperthermophilic Archaeon
- 656 Cloning and Analysis of 16 S-rRNA gene and Transcriptional Factor TF IID gene from Hyperthermophilic Archaeum strain KOD 1
- Gene Cloning and Function Analysis of Replication Factor C from Thermococcus kodakaraensis KOD1(Biochemistry & Molecular Biology)
- Gene Cloning and Polymerase Chain Reaction with Proliferating Cell Nuclear Antigen from Thermococcus kodakaraensis KOD1(Biochemistry & Molecular Biology)
- Isolation of a New Surfactin Producer Bacillus pumilus A-1,and Cloning and Nucleotide Sequence of the Regulator Gene , psf-1
- Protein Engineering of Penicillinase as Affinity Ligands for Bioprocessing
- Isolation and Characterization of Long-Chain-Alkane Degrading Bacillus thermoleovorans from Deep Subterranean Petroleum Reservoirs
- Family B DNA polymerase from a hyperthermophilic archaeon Pyrobaculum calidifontis : Cloning, characterization and PCR application(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Metal-Mediated Control of the Activity of Bacillus subtilis Superoxide Dismutase
- Modeling of Cephalosporin C Production and Its Application to Fed-batch Culture
- Regulaton of Cephalosporin C Produciton by Endogeneous Methionine in Cephalosporium acremonium
- Morphological Differenctitaion in Relation to Cephalosporin C Synthesis by Cephalosporium acremonium
- Effect of Glucose and Methionine Consumption Rates on Cephalosporin C Production by Cephalosporium acremonium
- Isolation and Application of Extremophiles.
- Increased Heat Resistance of Bacillus subtills Spores in Oil
- Use of a Tumbling-Defective Mutant of Escherichia coli for Evaluation of Pollutant Toxicity by Computer-Assisted Image Analysis of Bacterial Motion
- Analysis of a Negative Regulator, SoxR, for the Arthrobacter Sarosine Oxidase Gene
- Cloning and Sequencing of the Sarcosine Oxidase Gene from Arthrobacter sp. TE1826
- Highly Conservative Sequence in the Carboxyl Terminus of Sarcosine Oxidase is Important for Substrate Binding
- Cloning and Nucleotide Sequence of the Urea Amidolyase Gene from Candida utilis
- Gene Cloning and Characterization of Thermostable Peptidyl Prolyl cis-trans Isomerase (PPIase) from Bacillus stearothermophilus SIC1
- Isolation of a New Mixotrophic Bacterium Which Can Fix CO_2 and Assimilate Aliphatic and Aromatic Hydrocarbons Anaerobically
- Properties of Bacterial Corrosion of Stainless Steel and Its Inhibition by Protamine Coating
- Biological Threshold Values of Procaryotic Gene Expression Which Is Controlled by the DNA Inverted Repeat Sequence and the mRNA Secondary Structure
- Effect of Amino Acid Substitutions at the Active Center on the Activity of Bacillus stearothermophilus Alcohol Dehydrogenase
- Tumor Suppressive Monoclonal Antibody Belonging to the V_H 7183 Family Directed to the Oncodevelopmental Carbohydrate Antigen on Human Hepatocellular Carcinoma
- Killing of Bacillus subtilis by Cell Suicide through Autolysis Induction
- Isolatino and Characterization of Oxygen Sensitive Mutants of Escherichia coli
- Heat-Induced Aggregation and Interaction with Membranes of Cytoplasmic Proteins and the Suppressive Effect of Heat Shock Proteins in Escherichia coli Cells
- Binding ability of chitinase onto cellulose : an atomic force microscopy study
- Cloning and Nucleotide Sequence of the Gene Coding for a Subunit of the Respiratory NADH Dehydrogenase from Bacillus stearothermophilus
- Effect of Cultivation Temperatures on Thermophilic and Mesophilic Enzyme Gene Expression in Bacillus subtilis
- Characteristics of Thermostable Pullulanase from Bacillus stearothermophilus and the Nucleotide Sequence of the Gene
- A New Way of Stabilizing Recombinant Plasmids
- Adaptation Strategy of Thermophiles toward Hyperthermophily and Their Molecular Bases
- Molecular bases of thermophily in hyperthermophiles
- Cellular Toxicity of Cadmium Ions and Their Detoxification by Heavy Metal-Specific Plant Peptides, Phytochelatins, Expressed in Mammalian Cells.