A Simple and Efficient Method for High Fidelity PCR Cloning Using Antibody-neutralizing Technology(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
We introduce the TA cloning antibody method for the high-fidelity PCR product amplified by family B DNA polymerase without purification. This method uses antibodies and Thermus aquaticus (Taq) DNA polymerase. The antibodies can inhibit only the activity of family B DNA polymerase, and Taq can co-work for A-tailing. This method has nearly cloning efficiency to that of the PCR product of Taq.
- 社団法人日本農芸化学会の論文
- 2003-09-23
著者
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Esaka M
Hiroshima Univ. Hiroshima
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Esaka Muneharu
Graduate School Of Biosphere Science Hiroshima University
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NISHIYA Yoshiaki
Bio-division, Toyobo Co., Ltd.
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KITABAYASHI Masao
Graduate School of Biosphere Sciences, Hiroshima University, Kagamiyama
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Kitabayashi M
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.:graduate School Of Biosphere Science Hiroshima Un
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Kitabayashi Masao
Graduate School Of Biosphere Sciences Hiroshima University
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