Improvement of Reverse Transcription PCR by RNase H(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
An improvement in the method of the Reverse Transcription PCR (RT-PCR) using RNase H is proposed here. We succeeded in RT-PCR amplification against the full sequence of the coding region (8.9 kb) of the Insulin-like growth factor II receptor gene which has the area called the GC-block of about 90% GC contents at the 5' terminal. Furthermore, the RNase H treatment improved the sensitivity of RT-PCR amplification against a general target.
- 社団法人日本農芸化学会の論文
- 2003-11-23
著者
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Esaka Muneharu
Graduate School Of Biosphere Sciences Hiroshima University
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Esaka Muneharu
Graduate School Of Biosphere Science Hiroshima University
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KITABAYASHI Masao
Graduate School of Biosphere Sciences, Hiroshima University, Kagamiyama
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Kitabayashi Masao
Graduate School Of Biosphere Sciences Hiroshima University
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