Studies on the Utilization of Hydrocarbons by Microorganisms : (I) Production of Cytochrome c by Candida albicans in Hydtocarbon Fermentation : (Part I) Ptoduction of Yeast Cells from Hydrocarbons

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During the course of the studies on the ability of hydrocarbon utilization and cytochrome c production by several kinds of yeast, we found out that Candidaalbicans showed high cell-yield and high cytochrome c production. In this paper the effects of medium constituent, pH of culture medium, amd aeration on cell-yield of C. albicans were investigated. Addition of corn steep liquor and a detergent, Tween 85,shortened the period of fermentation, and maintenance of pH at 5.2-5.4 increased the cell yield. The yield of dry cells was 5.25g from 7.75g of n-hexadecane when the yeast was cultivated in the following medium ; n-hyxadecane 7.75g, NH_4NO_3 5.0g, KH_2PO_4 2.5g, MgSO_4・7H_2O 1.0g, FeCl_3・6H_2O 0.02g, corn steep liquor 1.0g, Tween 85 0.2ml and tap water 1000ml. The effect of aeration was observed in the absence of detergeny, but not in the presence of detergent, under the conditions tested.
公益社団法人日本生物工学会の論文
1967-12-25