Purification and Characterization of Pseudotropine Forming Tropinone Reductase from Hyoscyamus niger Root Cultures(Biological Chemistry)
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概要
- 論文の詳細を見る
A pseudotropine-forming tropinone reductase was extracted from root cultures of Hyoscyamus niger that produce the tropane alkaloids hyoscyamine and scopolamine. The enzyme stereospecifically reduces tropinone to pseudotropine, oxidizing NADPH. It has an approximate molecular weight of 84, 000 and a pH optimum between 5.8 and 6.25. The Km value for tropinone is 35.1 μmol/l and for NADPH 21.1 μmol/l. Substrate specificity was tested for NADPH and several tropinone analogues.
- 社団法人日本農芸化学会の論文
- 1988-10-23
著者
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Hashimoto Takashi
Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Yamada Yasuyuki
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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Yamada Yasuyuki
Research Center For Cell And Tissue Culture Kyoto University
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DRAGER Birgit
Research Center fo Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Drager Birgit
Research Center For Cell And Tissue Culture Kyoto University
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Hashimoto Takashi
Research Center For Cell And Tissue Culture Kyoto University
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Hashimoto Takashi
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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YAMADA Yasuyuki
Research Center for Cell and Tissue Culture, Kyoto University
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HASHIMOTO Takashi
Research and Education Center for Genetic Information, Nara Institute of Science and Technology
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YAMADA Yasuyuki
Research Center for Ceil and Tissue Culture, Faculty of Agriculture, Kyoto University
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