LuxS affects biofilm maturation and detachment of the periodontopathogenic bacterium Eikenella corrodens(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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Previously, we reported that biofilm formation of Eikenella corrodens is regulated by autoinducer-2 (AI-2), based on observations that biofilm-forming efficiency of ΔluxS mutant was greater than that of the wild type (Azakami et al., J. Biosci. Bioeng., 102, 110-117, 2006). To determine whether the AI-2 molecule affects biofilm formation directly, we added purified AI-2 to luxS mutant and wild-type E. corrodens and compared biofilm formations by using a static assay. Results indicated that biofilm formation in E. corrodens was enhanced by the addition of AI-2. We also compared the biofilms formed by flow cell system for the luxS mutant and the wild type by using scanning electron microscopy and confocal laser scanning microscopy. The number of viable bacteria in the luxS mutant biofilm was dramatically reduced and more sparsely distributed than that of the wild type, which suggested that AI-2 might enhance the mature biofilm. Conversely, further analysis by modified confocal reflection microscopy indicated that the wild-type biofilm was matured earlier than that of the luxS mutant, and became thinner and more sparsely distributed with time. These data suggest that LuxS may facilitate the maturation and detachment of biofilm in E. corroders.
- 公益社団法人日本生物工学会の論文
著者
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Ebisu Shigeyuki
Department Of Conservative Dentistry Osaka University Faculty Of Dentistry
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Noiri Yuichiro
Department Of Conservative Dentistry Tokushima University School Of Dentistry
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Karim Mohammad
Department Of Anatomy And Histology Faculty Of Veterinary Science Bangladesh Agricultural University
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Azakami Hiroyuki
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Matsunaga Tetsuro
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Kato Akio
Department Of Agricultural Chemistry Faculty Of Agriclture Yamaguchi University
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Asahi Yoko
Department Of Immunobiology Meiji Pharmaceutical University
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Azakami Hiroyuki
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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Matsunaga Tetsuro
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University:(Present office)Department of Microbiology, Kumamoto University
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Hisamoto Tatsunori
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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Karim Mohammad
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University:Department of Biotechnology and Genetic Engineering, Islamic University
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