Isolation and Characteristics of a Bacillus subtilis Mutant Tolerant to the Lytic Action of Sucrose Esters of Long-Chain Fatty Acids

概要

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A spontaneous mutant of Bacillus subtilis 168,SR3,tolerant to the autolysis-inducing action of sucrose monoesters of long-chain fatty acids, was isolated. It was shown that its susceptibility to the lytic action of sucrose esters and glycerol esters of short-chain fatty acids, fatty acids themselves, some surfactants, uncouplers of oxidative phosphorylation, and β-lactam antibiotics against the mutant strain was similar to that of the wild-type strain. In the absence of sucrose monoesters, no substantial differences were observed between the mutant and the wild-type strains in cell autolysis and autolysin activity. It was found that in the mutant the cellular uptake of the molecules of sucrose ester of palmitic acid was suppressed. Also, a protein having a molecular weight of 41 kDa was richer in the membrane of strain SR3 than that of 168. The tolerance of the mutant to the lytic action of the ester is suggested to be derived from a decrease in the amount of ester molecules transferred into the membrane, where the activity of autolytic enzymes may be controlled.
公益社団法人日本生物工学会の論文
1993-03-25