Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds : Color-based selection of ade1 or ade2 mutants(BREWING AND FOOD TECHNOLOGY)
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概要
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We identified Saccharomyces cerevisiae mutants with 100% higher intracellular glutathione using 1-methyl-3-nitro-1- nitrosoguanidine mutagenesis. This method employs visual selection of the most pigmented colonies among met30 strains carrying ade1 and ade2 mutations. Since the method does not involve genetic engineering, the mutants are suitable for use in the food industry.
- 2012-12-00
著者
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Nishiuchi Hiroaki
Ajinomoto Co. Inc
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Tarutina Marina
Russian State Collection of Industrial Microorganisms (VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms
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Dutova Tatiana
Russian State Collection of Industrial Microorganisms (VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms
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Yezhova Inna
Russian State Collection of Industrial Microorganisms (VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms
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Sineoky Sergey
Russian State Collection of Industrial Microorganisms (VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms
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- Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds : Color-based selection of colonies using the met30 strain
- Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds : Color-based selection of ade1 or ade2 mutants