Intracelluler Na^+ and K^+ Contents of Zygosaccharomyces rouxii Mutants Defective in Salt Tolerance

概要

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Two of five Zygosaccharomyces rouxii mutants defective in salt tolerance, 152S (sat1) and 1717S (SAT3), were inviable in a nutrient medium (YPD) containing more than 1% NaCl. These tow mutant cells contained significantly higher amounts of Na^+ (298 μmol and 285 μmol per g cells of 152S and 1717S, respectively) but lower amounts of K^+ (242 μmol and 176μmol per g cells of 152S and 1717S, respectively) than three other mutants, 41S (sat2-1[98 μmol Na^+ and 326 μmol K^+/g cells]), 197S(sat2-2{103μmol Na^+ and 336 μmol K^+/g cells]), 1611S(SAT4[139 μmol Na^+ and 294 μmol K^+/g cells]), as well as a wild-type strain, AN39(61 μmol Na^+ and 349 μmol K^+/g cells), when cultured in YPD medium containing 0.8% NaCl. A KCl supplement, optimally 0.6M, added to the medium somewhat restored the NaCl-hypersensitivity of 152S and 1717S with a concomitant decrease of intracellular Na^+. This finding suggests that the NaCl-hypersensitive mutations are due to a defect in the Na^+-regulating mechanism. The other three mutants showed weak responses to KCl in high NaCl-YPD. These five salt sensitive mutants and the wild-type strain retained the same levels of intracellular glycerol and arabitol when transferred into NaCl (5%)-YPD from YPD medium. This suggests that polyol accumulation is not the only mechanism of salt tolerance in Z. rouxii.
社団法人日本生物工学会の論文
1992-01-25