Sulfate Assimilatory Pathway Involving Bound Intermediates is Inoperative in Salmonella typhimurium(Biological Chemistry)

概要

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The existence of a carrier-bound pathway for inorganic sulfate assimilation has been proposed in Chlorella and Escherichia coli. The possibility that the sulfonyl group of active sulfate is transferred to a specific organic acceptor to from thiosulfate ester was examined with Salmonella typhimurium LT-2. Some 11% of the radioactive prducts from [^<35>S]-3'-phosphoadenosine 5'-phosphosulfate were transferred to high molecular weight compounds, and the remainder of the product is identified as free inorganic sulfite. Apparent thiosulfonate reductase activity was detected in the reaction mixtures containing S-sulfoglutathione and NADPH as conceivable substrates, but no with partially purified sulfite reductase. The former activity was attributable to the nonenzymatic reaction, sulfitolysis. Through these in vitro experiments the existence of the carrier-bound pathway was disproved.
社団法人日本農芸化学会の論文
1986-12-23