Reducing-agents-mediated Solubilization and Activation of Debranching Enzyme (Pullulanase) in Rice Flour
スポンサーリンク
概要
- 論文の詳細を見る
The effects of reducing agents on solubilization and activation of the debranching enzyme (pullulanase) were examined using rice flour. The activity of the debranching enzyme was observed in a buffer solution (pH 7.5) in which rice flour was incubated together with thiol-reducing reagents, (dithiothreitol, 2-mercaptoethanol etc.), but there was only low activity in the absence of reducing agents. Immunochemical measurement and the specific activity of the enzyme showed that the activation caused by the reductant was due to solubilization of the enzyme protein besides the enzyme activation.
- 社団法人日本農芸化学会の論文
- 1999-03-23
著者
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Yamaguchi Junji
Bioscience Center Nagoya Univ.
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Yamaguchi Junji
Biosci. Cent. & Grad. Sch. Bioagri. Sci. Nagoya Univ.
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Yamaguchi J
Sankyo Co. Ltd. Tokyo Jpn
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Masui Hironori
Mukogawa Women's University Junior College Division Department Of Dietary Life Science
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TAKEUCHI Wakako
Faculty of Domestic Science, Nagoya Women's University
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Takeuchi W
Faculty Of Domestic Science Nagoya Women's University
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Takeuchi Wakako
Faculty of Domestic Science, Nagoya Women's University
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