Biosynthesis of Vitamin B_6 in Rhizobium : In Vitro Synthesis of Pyridoxine from 1-Deoxy-D-xylulose and 4-Hydroxy-L-threonine(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Pyridoxine (vitamin B_6) in Rhizobium is synthesized from 1-deoxy-D-xylulose and 4-hydroxy-L-threonine. To define the pathway enzymatically, we established an enzyme reaction system with a crude enzyme solution of R. meliloti IFO14782. The enzyme reaction system required NAD^+ , NADP^+ , and ATP as coenzymes, and differed from the E. coli enzyme reaction system comprising PdxA and PdxJ proteins, which requires only NAD^+ for formation of pyridoxine 5'-phosphate from 1-deoxy-D-xylulose 5-phosphate and 4-(phosphohydroxy)-L-threonine.
- 社団法人日本農芸化学会の論文
- 2002-04-23
著者
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Hoshino Tatsuo
Departments of Medicine, Keio University and The Lankenau Hospital
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Hoshino T
Department Of Applied Microbiology Nippon Roche Research Center
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TAZOE Masaaki
Department of Applied Microbiology, Nippon Roche Research Center
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ICHIKAWA Keiko
Department of Applied Microbiology, Nippon Roche Research Center
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Tazoe Masaaki
Department Of Applied Microbiology Nippon Roche Research Center
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Hoshino Tatsuo
Department Of Applied Microbiology Nippon Roche Research Center
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Tazoe M
Department Of Applied Microbiology Nippon Roche Research Center
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Ichikawa Kimihisa
Department Of Applied Microbiology Nippon Roche Research Center
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