Cloning of the Pyridoxine 5'-Phosphate Phosphatase Gene (pdxP) and Vitamin B_6 Production in pdxP Recombinant Sinorhizobium meliloti
スポンサーリンク
概要
- 論文の詳細を見る
A novel gene (pdxP) encoding a pyridoxine 5′-phosphate (PNP) phosphatase involved in the last step of pyridoxine biosynthesis was cloned from Sinorhizobium meliloti IFO 14782 on the basis of the peptide sequences of the natural enzyme. The pdxP gene is an open reading frame (708 bp) encoding 235 amino acid residues with a calculated molecular weight of 26,466. From its deduced amino acid sequence, it was predicted that the enzyme belongs to the haloacid dehalogenase superfamily.Transformants of Escherichia coli and S. meliloti by pdxP gene expression plasmids showed stimulated PNP phosphatase activities. When pdxP was overexpressed together with the PNP synthase gene (pdxJ) in S. meliloti, the recombinant strain produced 149 mg/l of pyridoxine, 46% and 16% higher than the host strain and the pdxJ recombinant of S. meliloti respectively.
- 社団法人 日本農芸化学会の論文
- 2008-02-23
著者
-
Tazoe Masaaki
Department Of Applied Microbiology Nippon Roche Research Center
-
Hoshino Tatsuo
Department Of Applied Microbiology Nippon Roche Research Center
-
NAGAHASHI Yoshie
Department of Applied Microbiology, Nippon Roche Research Center
-
Nagahashi Yoshie
Department Of Applied Microbiology Nippon Roche Research Center
関連論文
- A NEW APPROACH TO NONIVASIVE CORONARY ARTERY VISUALIZATION USING CROSS SECTIONAL ECHOCARDIOGRAPHY : Echocardiography I : IIIrd Auditorium : Proceedings of the 43rd Annual Meeting of the Japanese Circulation Society, Tokyo, 1979
- Purification and Characterization of Pyridoxine 5'-Phosphate Phosphatase from Sinorhizobium meliloti
- Biosynthesis of Vitamin B_6 in Rhizobium : In Vitro Synthesis of Pyridoxine from 1-Deoxy-D-xylulose and 4-Hydroxy-L-threonine(Biochemistry & Molecular Biology)
- Production of Vitamin B_6 in Rhizobium
- Isolation and Characterization of a New Vitamin C Producing Enzyme (L-Gulono-γ-lactone Dehydrogenase) of Bacterial Origin
- Efficient Conversion of L-Sorbosone to 2-Keto-L-Gulonic Acid by Acetobacter liquefaciens Strains
- Metabolic Pathway for 2-Keto-L-gulonic Acid Formation in Gluconobacter melanogenus IFO 3293(Microbiology & Fermentation Industry)
- Microbial Production of 2-Keto-L-Gulonic Acid from L-Sorbose and D-Sorbitol by Gluconobacter melanogenus(Microbiology & Fermentation Industry)
- Purification and Properties of Membrane-bound D-Sorbitol Dehydrogenase from Gluconobacter suboxydans IFO 3255(Microbiology & Fermentation Technology)
- Purification and Properties of NADPH-Linked L-Sorbose Reductase from Gluconobacter melanogenus N44-1(Biological Chemistry)
- Isolation and Characterization of NAD(P)-Dependent L-Sorbosone Dehydrogenase from Gluconobacter melanogenus UV10(Microbiology & Fermentation Industry)
- Isolation and Characterization of Membrane-bound L-Sorbose Dehydrogenase from Gluconobacter melanogenus UV10(Microbiology & Fermentation Industry)
- Main Polyol Dehydrogenase of Gluconobacter suboxydans IFO 3255, Membrane-bound D-Sorbitol Dehydrogenase, That Needs Product of Upstream Gene, sldB, for Activity(Biochemistry & Molecular Biology)
- Molecular cloning and functional expression of D-sorbitol dehydrogenase from Gluconobacter suboxydans IFO3255, which requires pyrroloquinoline quinone and hydrophobic protein SldB for activity development in E.coli(Biochemistry & Molecular Biology)
- L-Sorbose Dissimilation in 2-Keto-L-gulonic Acid-producing Mutant UV10 Derived from Gluconobacter melanogenus IFO 3293(Microbiology & Fermentation Industry)
- RESISTANCE AND COMPLIANCE IN PULMONARY HYPERTENSION, WITH REFERENCE TO EFFECTS OF HYPOXIA AND ISOSORBIDE DINITRATE : Pulmonary Circulation, Peripheral Circulation : IVth Auditorium : Proceedings of the 43rd Annual Meeting of the Japanese Circulation Socie
- Isolation and Characterization of a New Quinoprotein Dehydrogenase, L-Sorbose/L-Sorbosone Dehydrogenase
- Development of a Stable Shuttle Vector and a Conjugative Transfer System for Gluconobacter oxydans
- Cloning of the Pyridoxine 5'-Phosphate Phosphatase Gene (pdxP) and Vitamin B_6 Production in pdxP Recombinant Sinorhizobium meliloti