Purification and Characterization of Isocitrate Lyase from Rhodopseudomonas sp. No.7(Microbiology & Fermentation Industry)

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概要

• 論文の詳細を見る

• Isocitrate lyase was purified from the purple nonsulfur bacterium Rhodopseudomonas sp. No. 7. The purified enzyme was electrophoretically homogeneous. The molecular weights of the native enzyme and its subunit were estimated to be approximate 250,000 and 62,000 by gel filtration chromatography and SDS-polyacrylamide gel electrophoresis, respectively. The optimum pH for its activity was 6.5. The optimum temperature was 45℃. The Km for DL-isocitrate was 0.136niM in potassium phosphate buffer (pH 6.0). Mg^<2+> was required for full activity of the enzyme as a non-essential activator. The enzyme activity was inhibited by SH-blocking reagents. Non-competitive inhibitory effects on the enzyme were examined with malate and succinate. The Ki for malate and succinate were 2.7 and 0.24 mM, respectively.

• 社団法人日本農芸化学会の論文

著者

• ANDO Akikazu

  Department of Biotechnology, Graduate School of Science and Technology, Chiba University

• Fujii T

  Kyoto Univ. Kyoto

• SHINOYAMA HIROFUMI

  Department of Bioresources Science, Graduate School of Science and Technology, Chiba University

• FUJII TAKAAKI

  Department of Bioresources Science, Graduate School of Science and Technology, Chiba University

• Ando A

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Ando Akikazu

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Ando Akikazu

  Department Of Agricultural Chemistry Chiba University

• TAHAMA Hirotsugu

  Department of Agricultural Chemistry, Chiba University

• Shinoyama H

  Department Of Advanced Bioresources Science Graduate School Of Science And Technology Chiba Universi

• Shinoyama Hirofumi

  Department Of Agricultural Chemistry Chiba University

• Fujii Takaaki

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Tahama Hirotsugu

  Department Of Bioproduction Science Chiba University

• FUJII Takaaki

  Department of Agricultural Chemistry, Chiba University

• Shinoyama Hirofumi

  Department of Advanced Bioresources Science, Graduate School of Science and Technology, Chiba University

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