Preparation of a Cell-free Ethylene-forming System from Penicillium digitatum
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概要
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An ethylene-forming enzyme was partially purified from Penicillium digitatum IFO 9372 by means of DEAE-Sepharose CL-6B ion exchange chromatography, and a cell-free ethylene-forming system was constructed. Using this system as a tool for elucidation of the biochemical basis of the ethylene formation, the immediate precursor of ethylene was found to be α-ketoglutarate and essential factors were L-arginine and the ferrous ion under reduced conditions. It was strongly suggested that this cell-free ethylene-forming system operates in living cells of P. digitatum on comparison of the time course of ethylene-forming activity of the cell-free system with that of living cells.
- 社団法人 日本農芸化学会の論文
著者
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Fujii Takao
Department of Applied Life Science, Faculty of Engineering, Sojo University
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OGAWA TAKAHIRA
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi
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Fujii T
Kyoto Univ. Kyoto
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FUKUDA HIDEO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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Fujii Takao
Department Of Applied Life Science Faculty Of Biotechnology And Life Science Sojo University
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Ogawa Takahira
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Fukuda Hideo
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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