Scale-Up of Glucoamylase Production by Saccharomycopsis fibuligera

概要

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The correlation between the yield of gluboamylase from cultures of Saccharomycopsis fibuligera IFO 0111 and the oxygen-transfer rate (OTR) was examined. There was an optimum OTR (30-35×10^<-7> gmol O_2/ml/min) for the production of glucoamylase, irrespective of the type of flask or jar fermentor (nominal volumes : 20-l, 100-l, 200-l and 4,000-l used. From experiments with a 100-l jar fermentor, we found that the optimum OTR resulted in the level of dissolved oxygen (DO falling to zero for only a few hours when cell growth reached a maximum. Moreover, it appeared necessary to maintain this optimum OTR even after the cell growth ceased. When production of glucoamylase was examined in a 4,000-l stirred-tank fermentor (pilot-plant scale) at the optimum OTR, the total amount of glucoamylase produced reached 61 U/ml, which was almost the same as that produced in flasks and in the 100-l jar fermentor.
社団法人日本生物工学会の論文
1993-11-25