Characterization of a Novel Polyphenol-Specific Oligoxyloside Transfer Reaction by a Family 11 Xylanase from Bacillus sp. KT12

概要

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A culture filtrate of Bacillus sp. KT12 was used to prepare polyphenyl β-oligoxylosides from xylan and polyphenols in a one-step reaction. One oligoxyloside transfer enzyme was purified from multiple xylanolytic enzymes in the culture filtrate. N-terminal amino acid sequence determination classified the enzyme as a glycosyl hydrolase family 11 (endo-xylanase). The xylanolytic enzyme activities could be markedly altered; its hydrolytic activity was almost entirely inhibited at acidic pH, whereas near constant transxylosylation activity was observed at pH 4–11. Further, metal ions activated transxylosylation and almost completely inhibited hydrolysis. The enzyme specifically induced a β-xylosyl transfer reaction to acceptor molecules, such as divalent and trivalent phenolic hydroxyl groups, and displayed no activity toward alcoholic compounds. The Bacillus sp. KT12 xylanolytic enzyme was a suitable enzyme for the synthesis of polyphenyl β-oligoxylosides.
社団法人日本農芸化学会の論文
2008-09-23