Identification of 2-(Cysteinyl)amido-2-deoxy-D-galacturonic Acid Residue from the Sheath of Leptothrix cholodnii

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概要

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• The sheath of Leptothrix cholodnii is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was S-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass spectrometry and NMR spectroscopy. The sheath was found to contain 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue.

• 社団法人 日本農芸化学会の論文
• 2006-05-23

著者

• SEKI Hiroko

  Chemical Analysis Center, Chiba University

• Koizumi Jun-ichi

  Div. Of Materials Sci. And Chemical Engineering Fac. Of Engineering Yokohama National Univ.

• Makita Hiroko

  Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokonama National Univ

• TAKEDA Minoru

  Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un

• KOIZUMI Jun-ichi

  Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un

• Katahira Masato

  Department Of Bioengineering Faculty Of Engineering Yokohama National University

• Katahira Masato

  Presto (precursory Res. For Embryonic Sci. Technology) Jst

• Nakahara Yuichi

  Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokonama National Univ

• FUKUI Hiroyuki

  Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National Un

• MIYANOIRI Yohei

  Department of Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama C

• Takeda Minoru

  Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ

• Koizumi Jun-ichi

  Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ

• Miyanoiri Yohei

  Department Of Supramolecular Biology International Graduate School Of Arts And Sciences Yokohama Cit

• Seki Hiroko

  Chemical Analysis Center Chiba Univ.

• Fukui Hiroyuki

  Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokonama National Univ

• Takeda Minoru

  Division Of Bioengineering Faculty Of Engineering Yokohama National University

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