A Novel Gene Encoding an Enzyme That Degrades a Polysaccharide from the Sheath of Sphaerotilus natans(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
A gene encoding an enzyme that is able to depolymerize the basic polysaccharide prepared from the sheath of Sphaerotilus natans was identified in a sheath-degrading bacterium, Paenibacillus koleovorans. The gene was constructed from 2217 bp coding for 738 amino acids, including the signal sequence of 34 amino acids. No closely related protein or gene was indicated by a homology search. The gene was expressed in Escherichia coli as a glutathione S-transferase fusion protein. The fusion protein depolymerized the sheath polysaccharide into an oligosaccharide, introducing an unsaturated sugar residue, suggesting that the gene codes for a polysaccharide lyase acting on a basic polysaccharide.
- 社団法人日本農芸化学会の論文
- 2003-10-23
著者
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Takeda M
Department Of Chemical And Energy Engineering Graduate School Of Engineering Yokohama National Unive
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Takeda Minoru
Storage Technologies Department Optical System Development Div. Henc Sony Corporation
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Koizumi J
Department Of Chemical And Energy Engineering Graduate School Of Engineering Yokohama National Unive
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TAKEDA Minoru
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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NISHINA Katsuya
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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HANAOKA Yuji
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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HIGO Motoko
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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TERUI Mutsumi
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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SUZUKI Ichiro
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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KOIZUMI Jun-ichi
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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Higo Motoko
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Hanaoka Yuji
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Terui Mutsumi
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Nishina Katsuya
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Takeda Minoru
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Koizumi Jun-ichi
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Suzuki Ichiro
Department Of Chemical And Energy Engineering Graduate School Of Engineering Yokohama National Unive
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Takeda Minoru
Division Of Bioengineering Faculty Of Engineering Yokohama National University
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Suzuki Ichiro
Division Of Materials Science And Chemical Engineering Graduate School Of Engineering Yokohama National University
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TAKEDA Minoru
Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University
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TAKEDA Minoru
Department of Physics, Tokai University
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