Structural Analysis of the Extracellular Polysaccharide Produced by Sphaerotilus natans(Biochemistry & Molecular Biology)
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概要
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An extracellular polysaccharide (EPS) was recovered and purified from the culture fluid of a sheathed bacterium, Sphaerotilus natans. Glucose, rhamnose, and aldobiouronic acid were detected in the acid hydrolysate of EPS by thin-layer chromatography (TLC). The aldobiouronic acid was found to be composed of glucuronic acid and rhamnose by TLC and gas-liquid chromatography analyses of the corresponding neutral disaccharide. The structure of EPS was identified by methylation linkage analysis and nuclear magnetic resonance. Additionally, partial acid hydrolysates of EPS were prepared and put through fast atom bombardment-mass spectrometry to determine the sugar sequence of EPS. The resulting data showed that EPS produced by S. natans is a new gellan-Iike polysaccharide constructed from a tetrasaccharide repeating unit, as shown below. →4)-α-D-Glcp-(1→2)-β-D-glcAp-(1→2)-α-L-Rhap-(1→3)-β-L-Rhap-(1→
- 社団法人日本農芸化学会の論文
- 2002-07-23
著者
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Takeda M
Department Of Chemical And Energy Engineering Graduate School Of Engineering Yokohama National Unive
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Takeda Minoru
Storage Technologies Department Optical System Development Div. Henc Sony Corporation
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Koizumi J
Department Of Chemical And Energy Engineering Graduate School Of Engineering Yokohama National Unive
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TAKEDA Minoru
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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KOIZUMI Jun-ichi
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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NOMOTO Shogo
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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Nomoto Shogo
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Takeda Minoru
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Koizumi Jun-ichi
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Takeda Minoru
Division Of Bioengineering Faculty Of Engineering Yokohama National University
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TAKEDA Minoru
Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University
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TAKEDA Minoru
Department of Physics, Tokai University
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