Cloning and Expression of the Gene Encoding Thermostable Poly(3-Hydroxybutyrate) Depolymerase
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概要
- 論文の詳細を見る
The gene encoding extracellular poly(3-hydroxybutyrate) depolymerase from a thermophilic poly(3-hydroxybutyrate)-degrading bacterium, strain HS, was cloned and intracellularly expressed in Escherichia coli. The gene was found to consist of 1485-bp nucleotide sequence coding for a 22-amino-acid signal peptide and a 473-amino-acid mature protein. Phylogenetic analysis and domain structure showed that the enzyme was clustered with type II PHB depolymerases. The gene was expressed in E. coli under the controlof the tac promoter. A 46-kDa protein was detected in the cell extract by SDS-Page. The N-terminal sequence of the protein agreed with that of the original enzyme. The crude enzyme was able to degrade PHB particles at 70℃.
- 社団法人日本生物工学会の論文
- 2000-10-25
著者
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Adachi Kenji
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Takeda M
Department Of Chemical And Energy Engineering Graduate School Of Engineering Yokohama National Unive
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Takeda Minoru
Storage Technologies Department Optical System Development Div. Henc Sony Corporation
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Koizumi J
Department Of Chemical And Energy Engineering Graduate School Of Engineering Yokohama National Unive
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TAKEDA Minoru
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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HANAOKA Yuji
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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SUZUKI Ichiro
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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KOIZUMI Jun-ichi
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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KITASHIMA KOUICHIRO
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un
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Hanaoka Yuji
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Takeda Minoru
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Koizumi Jun-ichi
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Suzuki Ichiro
Department Of Chemical And Energy Engineering Graduate School Of Engineering Yokohama National Unive
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Kitashima Kouichiro
Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ
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Takeda Minoru
Division Of Bioengineering Faculty Of Engineering Yokohama National University
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Suzuki Ichiro
Division Of Materials Science And Chemical Engineering Graduate School Of Engineering Yokohama National University
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TAKEDA Minoru
Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University
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TAKEDA Minoru
Department of Physics, Tokai University
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