Isolation and Chemical Composition of the Sheath of Sphaerotilus natans

スポンサーリンク

概要

• 論文の詳細を見る

• A sheathed bacterium, Sphaerotilus natans, was cultured with vigorous shaking in a medium containing peptone. Then the biomass was harvested and treated with lysozyme, sodium dodecyl sulfate, and protease. With treatment, 1.6 mg of sheaths was obtained from 15 mg of biomass. For the preparation of sheaths of high purity, cultivation must be in the absence of glucose with sufficient aeration to prevent poly (3-hydroxybutyrate) accumulation. Carbohydrate (54.1%), protein (12.2%), and lipid (1-3%) were detected in the sheaths by colorimetric reactions and solvent extraction. Gas-liquid chromatography showed glucose and galactosamine to be present in the molar ratio of 1 : 4. The most abundant amino acids in the sheath protein were glycine (49.2 mol%) and cysteine (24.6 mol%). The sheaths were resistant to agents that reduce disulfide bonds (dithiothreitol and 2-mercaptoethanol) and to protease. However, sheathes were degraded completely by hydrazine, and a heteropolysaccharide composed of glucose and galactosamine (1 : 4) was released. The weight-average molecular weight of the polysaccharide was estimated to be 1.2×10^5 by gel filtration chromatography with a low-angle laser-light scattering photometer and a rotation index detector. A ladder of 1.5-kDa peptides separable by sodium dodecyl sulfate gel electrophoresis was obtained by partial hydrolysis of sheaths, suggesting the sheath protein has repeating units of 1.5 kDa.

• 社団法人日本農芸化学会の論文
• 1998-06-23

著者

• Nakano Fumio

  Division Of Bioengineering Faculty Of Engineering Yokohama National University

• Takeda M

  Department Of Chemical And Energy Engineering Graduate School Of Engineering Yokohama National Unive

• Koizumi J

  Department Of Chemical And Energy Engineering Graduate School Of Engineering Yokohama National Unive

• Takeda Minoru

  Department Of Chemical And Energy Engineering Graduate School Of Engineering Yokohama National Unive

• TAKEDA Minoru

  Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National Un

• NAGASE Tomomi

  Division of Bioengineering, Faculty of Engineering Yokohama National University

• IOHARA Keishi

  Division of Bioengineering, Faculty of Engineering Yokohama National University

• KOIZUMI Junichi

  Division of Bioengineering, Faculty of Engineering Yokohama National University

• Nagase T

  Division Of Bioengineering Faculty Of Engineering Yokohama National University

• Iohara Keishi

  Division Of Bioengineering Faculty Of Engineering Yokohama National University:(present Address)depa

• Koizumi Junichi

  Division Of Bioengineering Faculty Of Engineering Yokohama National University

• Takeda Minoru

  Division Of Materials Science And Chemical Engineering Faculty Of Engineering Yokohama National Univ

• Nagase Tomomi

  Division Of Bioengineering Faculty Of Engineering Yokohama National University

• Takeda Minoru

  Division Of Bioengineering Faculty Of Engineering Yokohama National University

関連論文

• High-Density Near-Field Optical Disc Recording
• EB Mastering Process for SIL Readout/Recording System(Nano-Fabrication and Patterned Media)
• Deep UV Mastering with a Write Compensation Technique Realizing over 20GB/layer Capacity Disc
• Deep UV Mastering Using an All-Solid-State 266 nm Laser for an over 20 GBytes/Layer Capacity Disk
• Quantum Theory of Massive Yang-Mills Fields. II : Gauge Covariance
• Quantum Theory of Massive Yang-Mills Fields. I : Basis of Formulation with Indefinite Metric
• Gauge Covariance in Non-Abelian Gauge Theories
• On Finite Local Gauge Transformations in a Yang-Mills Field Theory with Invariant Gauge-Families
• EN-P32 Bacterial diversity and mechanism of drinking water treatment using bio-filtration for removal of iron and manganese from groundwater(Section VI Environmental Biotechnology)
• Removal of Mn^ from water by "aged" biofilter media : The role of catalytic oxides layers(ENVIRONMENTAL BIOTECHNOLOGY)
• A Novel Gene Encoding an Enzyme That Degrades a Polysaccharide from the Sheath of Sphaerotilus natans(Biochemistry & Molecular Biology)
• Structural Analysis of the Extracellular Polysaccharide Produced by Sphaerotilus natans(Biochemistry & Molecular Biology)
• Commensal Relationship Between a Sheath-forming Bacterium, Sphaerotilus natans, and a Sheath-degrading Bacterium, Paenibacillus sp.(Microbiology & Fermentation Technology)
• Cloning and Expression of the Gene Encoding Thermostable Poly(3-Hydroxybutyrate) Depolymerase
• Isolation and Chemical Composition of the Sheath of Sphaerotilus natans
• Thermostable Poly(3-hydroxybutyrate)Depolymerase of a Thermophilic Strain of Leptothrix sp.Isolated from a Hot Spring
• Factors Affecting the Activity of a Protein Bioflocculant Produced by Nocardia amarae
• Identification of 2-(Cysteinyl)amido-2-deoxy-D-galacturonic Acid Residue from the Sheath of Leptothrix cholodnii
• Structural Analysis of the Fundamental Polymer of the Sheath Constructed by Sphaerotilus natans
• Deformation of the Liquid Oxygen Meniscus Induced by Magnetic Field
• Identification and Characterization of a Mycobacterial (2R,3R)-2,3-Butanediol Dehydrogenase
• A Spatial Relationship between Sheath Elongation and Cell Proliferation in Sphaerotilus natans

もっと見る 閉じる

スポンサーリンク