Dimerization and DNA Binding Facilitate .ALPHA.-Helix Formation of Max in Solution.
スポンサーリンク
概要
- 論文の詳細を見る
Max is a basic region/helix-loop-helix/leucine zipper (b/HLH/Z) protein that forms a hetero-complex with the Myc family proteins Myc, Mad, and Mxil, and a homo-complex with itself. These complexes specifically bind to double-stranded DNA containing CACGTG sequences. Here, we report on the structural properties in aqueous solution of a 109-aminoacid protein, Max110, corresponding to the N-terminal domain of Max containing the b/ HLH/Z motif (residues 2-110), as characterized by combined use of circular dichroism (CD) and sedimentation equilibrium experiments. The results showed that the α-helical content of Max 110 increases with increasing protein concentration. The sedimentation equilibrium data indicated that Max110 exists as a monomer at low protein concentration, and forms a dimer at high protein concentration. Further increases in the α-helical content of Max110 occur upon addition of DNA with the CACGTG recognition sequence. Thus, dimerization and binding to DNA of Max both favor an increase of the α-helical content.
- 社団法人 日本生化学会の論文
著者
-
Maeda Tadakazu
Department Of Physics Faculty Of Science Nagoya University:mitsubishi-kasei Institute Of Life Scienc
-
Uesugi Seiichi
Department Of Environment And Natural Sciences Graduate School Of Environment And Information Scienc
-
Katahira Masato
Department Of Bioengineering Faculty Of Engineering Yokohama National University
-
Kurihara Yasuyuki
Department Of Environment And Natural Sciences Graduate School Of Environment And Information Scienc
-
Saito Toshiyuki
Division Of Animal Science National Institute Of Agrobiological Sciences
-
Horiuchi Masataka
Department of Bioengineering, Faculty of Engineering, Yokohama National University
関連論文
- BIOCHEMICAL STUDY OF PANCREATIC PROTEINS IN THE RDW RAT WITH HEREDITARY HYPOTHYROIDISM
- Dynamic changes in intranuclear and subcellular localizations of mouse Prrp/DAZAP1 during spermatogenesis : the necessity of the C-terminal proline-rich region for nuclear import and localization
- Room Temperature Phosphoresesnce Study of Refolding of Disulfide Reduced RNase T_1
- Quasi-Elastic Light Scattering from Muscle F-Actin
- Isolation of a sex chromosome-specific DNA sequence in the medaka, Oryzias latipes
- Searching for new biomarkers of bladder cancer based on proteomic analysis
- 2P445 High-molecular-mass proteome analysis of prostate cancer by agarose 2-DE and LC-MS/MS(48. Bioinformatics, genomics and proteomics (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- ABNORMAL THYROGLOBULIN IN THE RDW RAT THYROID GLAND WITH HEREDITARY HYPOTHYROIDISM
- Protein carbonyl detection by two-dimensional fluorescence difference gel electrophoresis
- A simple method for peptide purification as a basis for peptidome analysis
- Random genomic clones as a tool to construct genetic map in Japanese medaka, Orizias latipes
- Correlation among Secondary Structure, Amyloid Precursor Protein Accumulation, and Neurotoxicity of Amyloid β(25-35) Peptide as Analyzed by single Alanine Substitution
- Hairpin Structure of an RNA 28-mer, Which Contains a Sequence of the Enzyme Component of a Hammerhead Ribozyme System: Evidence for Tandem G: A Pairs That Are Not of Side-by-Side Type^1
- Properties of Hepatitis Delta Virus Ribozyme, Which Consists of Three RNA Oligomer Strands^1
- PE-052 Three-dimensional Modeling of Two-stent Deployment at Left Main Coronary Artery Bifurcation Using Micro-focus Computed Tomography(Coronary revascularization, PCI(07)(IHD),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Ci
- Identification of 2-(Cysteinyl)amido-2-deoxy-D-galacturonic Acid Residue from the Sheath of Leptothrix cholodnii
- Ductal carcinoma in situ : correlations between high-resolution magnetic resonance imaging and histopathology
- Dimerization and DNA Binding Facilitate α-Helix Formation of Max in Solution
- RNA Recognition Mechanism of the Minimal Active Domain of the Human Immunodeficiency Virus Type-2 Nucleocapsid Protein
- Structural Analysis of the Fundamental Polymer of the Sheath Constructed by Sphaerotilus natans
- Hyperglycemia-induced Alteration in Proteomes of the Rat Renal Cortex
- Profilin 1, overexpression in renal cell carcinoma
- Platelets prevent acute liver damage after extended hepatectomy in pigs
- Structure, Interactions and Effects on Activity of the 5'-terminal Region of Human telomerase RNA
- Activation and Repression of the Activity of a Lead Ribozyme by the Combination of Pb^ and Mg^
- Properties of a Hammerhead Ribozyme with Deletion of STem II^1
- Three-Dimensional Solution Structure of SGTx1, a Voltage-gated Potassium Channels Antagonist
- Vertical fissure : computed tomographic observation and correlation with chest radiography
- ACCUMULATION OF MOLECULAR CHAPERONE PROTEINS IN THE ENDOPLASMIC RETICULUM FROM THE rdw RAT THYROID CELLS WITH HYPOTHYROIDISM
- CHARACTERIZATION AND IDENTIFICATION OF PROTEINS RELATED TO THE RDW RAT WITH HEREDITARY DWARFISM
- A case of a fibroadenoma coexisting with an invasive lobular carcinoma in the breast
- Mechanism of mammalian germ cell development : toward assisted new reproductive technologies
- Severity-based treatment for Japanese patients with MPO-ANCA-associated vasculitis : the JMAAV study
- Spectra and Mean Association Number of Porphyrin J Aggregate
- Dimerization and DNA Binding Facilitate .ALPHA.-Helix Formation of Max in Solution.