Searching for new biomarkers of bladder cancer based on proteomic analysis

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概要

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• Urine cytology is the gold standard for detection of bladder cancer. However, it has poor sensitivity, and currently several diagnostic markers have high false-positive rates. One of the problems with bladder cancer is high locoregional recurrence after surgery. Therefore new diagnostic markers of bladder cancer are needed. We have conducted a comprehensive study of high molecular mass (HMM) protein expression in bladder cancer tissue by means of agarose two-dimensional gel electrophoresis (agarose 2-DE) followed by the analysis of liquid chromatography tandem mass spectroscopy. We successfully identified 53 proteins from cancer tissue and 33 proteins from normal mucosa in the HMM area of the 2DE gel, and they were classified as proteins of transcription, translation or the cytoskeleton. Among them, eight differentially regulated proteins were selected as new marker candidates, and we performed experiments to validate their use as diagnostic markers. The results of Western blotting showed that these proteins except for α spectrin were significantly upregulated in bladder cancer tissue, and one of them, structural maintenance of chromosomes protein 3 (SMC3), was specifically detected in urine samples from bladder cancer patients. On the other hand, the results of Immunohistochemistry showed that expression of neuroblast differentiation-associated protein AHNAK (AHNAK) was significantly increased and that of plectin-1 was significantly decreased in the bladder cancer specimens compared with normal bladder mucosa specimens. However, no significant difference was observed in other proteins. These results suggest that AHNAK and Plectin-1 would be the potential markers for urine cytology and SMC3 is possible to be that for diagnosis marker for urinalysis.

• 日本電気泳動学会の論文
• 2008-03-01

著者

• Matsumoto Kazumasa

  Department Of Urology Kitasato University School Of Medicine

• Matsumoto Kazumasa

  北里大学 医学部泌尿器科学

• Baba Shiro

  Department of Pediatrics, Graduate School of Medicine, Kyoto University

• Baba S

  Department Of Urology Kitasato University School Of Medicine

• Baba Shiro

  北里大学 医学部泌尿器科学

• Baba Shiro

  Department Of Pediatrics Graduate School Of Medicine Kyoto University

• Satoh Takefumi

  北里大学 医学部泌尿器科学

• Satoh Takefumi

  Department Of Urology Kitasato University

• Suyama Takahito

  Department Of Urology Graduate School Of Medicine Chiba University

• Mikami Kazuo

  Department Of Urology Chibaken Saiseikai Narashino Hospital

• IWAMURA Masatsugu

  Department of Urology, Kitasato University School of Medicine

• OKUSA Hiroshi

  Department of Urology, Kitasato University School of Medicine

• Okusa Hiroshi

  Department Of Urology Kitasato University School Of Medicine

• Matsushita Kazuhito

  Department Of Radiology Kitasato University School Of Medicine

• Iwamura Masatsugu

  北里大学 医学部泌尿器科学

• OH-ISHI Masamichi

  Department of Physics, School of Science

• MAEDA Tadakazu

  Department of Physics, School of Science

• Oh-ishi Masamichi

  Department Of Physics Kitasato University School Of Science

• Maeda Tadakazu

  Laboratory Of Biomolecular Dynamics Department Of Physics Kitasato University School Of Science

• Maeda Tadakazu

  Graduate School Of Science Kitasato University

• Maeda Tadakazu

  Department Of Physics Faculty Of Science Nagoya University:mitsubishi-kasei Institute Of Life Scienc

• Kitano Masashi

  Department Of Radiology Kitasato University School Of Medicine

• Miki Kenta

  Department Of Urology Jikei University School Of Medicine

• KODERA Yoshio

  Departments of Physics, Kitasato University School of Science

• MINAMIDA Yutaka

  Department of Urology, Kitasato University, School of Medicine

• TSUCHIDA Mayumi

  Department of Urology, Kitasato University, School of Medicine

• KAVOUSSI Nicholas

  Department of Applied Physics and Applied Mathematics, Columbia University School of Engineering and

• SATO Takefumi

  Department of Urology, Kitasato University, School of Medicine

• Saika Takashi

  川崎医科大学 泌尿器科学

• Kato Masao

  Department Of Urology Division Of Frontier Medical Science Programs For Biomedical Research Graduate

• Mineta Kaori

  Department Of Urology School Of Medicine University Of Occupational And Environmental Health

• Baba Shiro

  Department Of Urology Kitasato University

• Baba Shiro

  Departments Of Urology Kitasa To University School Of Medicine

• SHITARA TOSHIYA

  Fuchinobe General Hospital

• Kodera Yoshio

  Laboratory Of Biomolecular Dynamics Department Of Physics Kitasato University School Of Science

• Saka Toshihisa

  Department Of Urology Nara Medical University

• Tatarano Shuichi

  Department Of Urology Graduate School Of Medical And Dental Sciences Kagoshima University

• Matsumoto Kazumasa

  Department Of Urology Kitasato University Hospital

• Iwamura Masatsugu

  Department Of Urology Kitasato University Hospital

• Satoh Takefumi

  Department Of Urology Kitasato University Hospital

• Kavoussi Nicholas

  Department Of Applied Physics And Applied Mathematics Columbia University School Of Engineering And

• Minamida Yutaka

  Department Of Urology Kitasato University School Of Medicine

• Tsuchida Mayumi

  Department Of Urology Kitasato University School Of Medicine

• Kasahara Masato

  Department Of Medicine And Clinical Science Kyoto University Graduate School Of Medicine

• Matsui Takashi

  Department Of Physics School Of Science Kitasato University

• Miki Kenta

  Department Of Urotogy Jikei University School Of Medicine

• MATSUDA Kenji

  Japanese Society of Chemotherapy (JSC)

• Kodera Yoshio

  Department Of Physics Kitasato University School Of Science

• Maeda Tadakazu

  Department Of Physics Kitasato University School Of Science

• Kawahara Motoshi

  Department Of Urology Kagoshima University Hospital

• Kobayashi Masao

  Department Of Radiology Division Of Radiotherapy The Jikei University School Of Medicine.

• 小林 雅夫

  Department Of Radiology The Jikei University School Of Medicine

• Kondo Mitsuko

  First Department Of Medicine Tokyo Women's Medical University School Of Medicine

• Mutaguchi Kazuaki

  Department Of Urology Hiroshima University

• Kinuta Masahiro

  岡山大学 医歯総合研究

• KAJI Masayuki

  Shizuoka City Public Health Center

• KAWAKAMI Masahiko

  Ryokufuso Hospital

• Baba Shiro

  Department Of Neurosurgery Nagasaki University Graduate School Of Biomedical Sciences

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