16S rRNA Gene-Based Analysis of Microbial Community by Whole-Genome Amplification and Minigel-Single-Strand Conformation Polymorphism Technique(METHOD)
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概要
- 論文の詳細を見る
We have developed an analytical technique for the 16S rRNA gene that comprises whole-genome amplification and the polymerase chain reaction (PCR)-minigel-single-strand conformation polymorphism technique (WGA-SSCP). Under optimal conditions, SSCP bands could be detected when genomic DNA from bacteria of interest comprised 0.5% or more of the specimen. This method will be effective for the identification of nonculturable bacteria in a microbial community.
- 社団法人日本生物工学会の論文
- 2006-11-25
著者
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SHINOYAMA HIROFUMI
Department of Bioresources Science, Graduate School of Science and Technology, Chiba University
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OTO MICHIEI
Department of Biotechnology, Tokyo Technical College
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Oto Michiei
Department Of Biotechnology Tokyo Technical College
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Suda Wataru
Graduate School Of Horticulture Chiba Univ.
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Suda Wataru
Department of Biotechnology, Tokyo Technical College
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Shinoyama H
Department Of Advanced Bioresources Science Graduate School Of Science And Technology Chiba Universi
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Shinoyama Hirofumi
Department Of Agricultural Chemistry Chiba University
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Shinoyama Hirofumi
Department of Advanced Bioresources Science, Graduate School of Science and Technology, Chiba University
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