A Direct Method to Isolate DNA from Phyllosphere Microbial Communities without Disrupting Leaf Tissues

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概要

• 論文の詳細を見る

• We developed a method for <direct>___- D__-NA i__-solation from p__-hyllosphere microbial communities, designated Direct-DIP. This method comprises DNA extraction from non-shredded leaves with benzyl chloride, and DNA purification by gel filtration. Scanning electron microscopy showed that epiphytic microorganisms were completely removed from the leaf surface after benzyl chloride treatment, while microstructures of the leaf were not damaged. Clear DGGE profiles were obtained regardless of the plant species. Shannon diversity indices of DGGE profiles by Direct-DIP were higher than those by a conventional method. Our findings suggest that Direct-DIP is a rapid, simple, and cost-effective method of extracting DNA from phyllosphere microbial communities.

• 日本微生物生態学会の論文

著者

• Amachi Seigo

  Graduate School Of Horticulture Chiba University

• Shishido Masahiro

  Graduate School Of Horticulture Chiba Univ.

• SUDA WATARU

  Graduate School of Horticulture, Chiba University

• OTO MICHIEI

  Department of Biotechnology, Tokyo Technical College

• SHINOYAMA HIROFUMI

  School of Art and Design, Meisei University

• Oto Michiei

  Department Of Biotechnology Tokyo Technical College

• Suda Wataru

  Graduate School Of Horticulture Chiba Univ.

• Shinoyama H

  Department Of Advanced Bioresources Science Graduate School Of Science And Technology Chiba Universi

• Shinoyama Hirofumi

  School Of Art And Design Meisei University

• Suda Wataru

  Graduate School Of Frontier Sciences The University Of Tokyo

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