Some Properties and Physiological Roles of Phosphoenolpyruvate Carboxylase in Rhodopseudomonas sp. No. 7 Grown on Ethanol under Anaerobic-light Conditions
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概要
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Phosphoenolpyruvate carboxylase (EC 4.1.1.31, PEPC) and phosphoenolpyruvate kinase (EC 4.1.1.49<ATP>, PEPK) were detected in cells of Rhodopseudomonas sp. No. 7 grown photoanaerobically on ethanol and acetate. The activity of PEPC was about 3 times higher in the ethanol-grown cells than the acetate-grown cells. PEPC was purified as an electrophoretically homogeneous and stable protein (Mr, about 400,000; subunit Mr, about 102,000). The enzyme absolutely required Mn^<2+> or Mg^<2+> for the appearance of its activity. Acetyl CoA (40 μM) reduced the K_m against phosphoenolpyruvate and HCO^-_3 to about 1/20 (0.23mM) and 1/2 (1.7mM), respectively. At that time, the V_<max> increased to about 30 times (90 μmol/min/mg protein). In the presence of acetyl CoA the circular dichroism spectrum of the enzyme showed the decrease of an α-helix structure. The enzyme was not activated by fructose-1, 6-bisphosphate. The enzyme was inhibited by aspartate (K_i, 0.208mM). Besides, the enzyme was inhibited by nucleotides (ATP and GTP). The enzyme activity was activated to about 15 times by 3.25M ethanol.
- 社団法人日本農芸化学会の論文
- 1997-04-23
著者
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Shinoyama Hirofumi
Department Of Bioproduction Science Faculty Of Horticulture Chiba University
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Shinoyama Hirofumi
Department Of Agricultural Chemistry Chiba University
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Suzuki Tomoaki
Department Of Bioproduction Science Faculty Of Horticulture Chiba University
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Fujii Takaaki
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Sadaie Megumi
Department Of Bioproduction Science Faculty Of Horticulture Chiba University
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NAGANO Takanari
Department of Bioproduction Science, Faculty of Horticulture, Chiba University
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Nagano Takanari
Department Of Bioproduction Science Faculty Of Horticulture Chiba University
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FUJII Takaaki
Department of Agricultural Chemistry, Chiba University
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Shinoyama Hirofumi
Department of Advanced Bioresources Science, Graduate School of Science and Technology, Chiba University
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