Production of Functional Lectin in Pichia pastoris Directed by Cloned cDNA from Aleuria aurantia(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
A plasmid bearing a nucleotide sequence of fucosespecific lectin of Aleuria aurantia was constructed and expressed in a methylotrophic yeast, Pichia pastoris. The product showed almost the same hemagglutinating activity as the lectin produced in Escherichia coli, the properties of which were quite similar to the native one. Because of glycosylation of the product, the molecular mass was larger than that of the native one, and it acquired higher thermostability.
- 社団法人日本農芸化学会の論文
- 2003-10-23
著者
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Amano Koh
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Ando A
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Nagata Y
Department Of Biotechnology University Of Tokyo
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Nagata Yuji
Department Of Applied And Environmental Chemistry Kitami Institute Of Technology
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Ando Akikazu
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Ando Akikazu
Department Of Agricultural Chemistry Chiba University
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Nagata Yoshiho
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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TAKASE Mitsuyo
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
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Takase Mitsuyo
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Nagata Y
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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