The lin Genes for γ-Hexachlorocyclohexane Degradation in Sphingomonas sp. MM-1 Proved to Be Dispersed across Multiple Plasmids

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概要

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• A γ-hexachlorocyclohexane (HCH)-degrading bacterium, Sphingomonas sp. MM-1, was isolated from soil contaminated with HCH isomers. Cultivation of MM-1 in the presence of γ-HCH led to the detection of five γ-HCH metabolites, γ-pentachlorocyclohexene, 2,5-dichloro-2,5-cyclohexadiene-1,4-diol, 2,5-dichlorohydroquinone, 1,2,4-trichlorobenzene, and 2,5-dichlorophenol, strongly suggesting that MM-1 has the lin genes for γ-HCH degradation originally identified in the well-studied γ-HCH-degrading strain Sphingobium japonicum UT26. Southern blot, PCR amplification, and sequencing analyses indicated that MM-1 has seven lin genes for the conversion of γ-HCH to β-ketoadipate (six structural genes, linA to linF, and one regulatory gene, linR). MM-1 carried four plasmids, of 200, 50, 40, and 30 kb. Southern blot analysis revealed that all seven lin genes were dispersed across three of the four plasmids, and that IS6100, often found close to the lin genes, was present on all four plasmids.

著者

• Tsuda Masataka

  Department Of Environmental Life Sciences Graduate School Of Life Sciences Tohoku University

• Tsuda Masataka

  Department Of Environmental Life Science Graduate School Of Life Science Tohoku University

• NAGATA Yuji

  Department of Biotechnology, University of Tokyo

• Ohtsubo Yoshiyuki

  Department of Biotechnology, The University of Tokyo

• Ohtsubo Yoshiyuki

  Department Of Biotechnology The University Of Tokyo

• Nagata Yuji

  Department Of Applied And Environmental Chemistry Kitami Institute Of Technology

• Ito Michihiro

  Department Of Engineering Science Hokkaido University

• Ito Michihiro

  Department Of Electrical Engineering Ii Faculty Of Engineering Kyoto University

• ENDO Ryo

  Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University

• TABATA Michiro

  Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University

• KUMAR Ashwani

  Environmental Biotechnology Section, Industrial Toxicology Research Center

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