Sulfide Oxidation by Gene Expressions of Sulfide-Quinone Oxidoreductase and Ubiquinone-8 Biosynthase in Escherichia coli

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概要

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• Sulfides (S^<2-> HS^-) such as hydrogen sulfide belong to a class of sulfur compounds with unpleasant odors. In order to confer sulfide-oxidizing ablity on the intestine-inhabiting bacteria, the sulfide-quinone oxidoreductase gene (sqr) in Rhodobacter capsulatus DSM-155 and genes for quinone biosynthesis (ubiC, ubiA and ispB) in Escherichia coli XL1 Blue-MRF' were transduced into E. coli BL21 (DE3). Plasmids pT7-7 and pSTV were used as vectors of sqr, and ubiCA and ispB, respectively. The recombinants sqr-BL21 (DE3) and ubiCA, ispB-sqr-BL21 (DE3) were successfully constructed. The maximal sulfide-removing activities of the whole cells and membrane fractions of sqr-BL21 (DE3) attained at pH8.0 and 7.8, were 267 nmol/mg cells (dry weight)/min and 1250 nmol/mg membrane fraction (protein)/min, respectively. The molecular ratio of sulfide (S^<2->) oxidized and oxygen (O_2) consumed was 2:1. SQR activity in the recombinant cells was positively restricted under anaerobic conditions and also by the addition of electron transfer inhibitors. Ubiquinone-8 (UQ-8) biosynthesis in the cells of ubiCA, ispB-sqr-BL21 (DE3) increased as much as 2.2-fold compared with that of (pSTV)-sqr-BL21 (DE3) during the 12-16 h incubation period. The maximal sulfide removal in the quinone-raised E. coli was attained slightly earlier, however, SQR activities thereafter were lower than those in (pSTV) -sqr-BL21 (DE3).

• 社団法人日本生物工学会の論文
• 1999-09-25

著者

• Takahashl Mika

  Laboratory Of Animal Environment Meiji University

• Takahashi Masaya

  Industrial Chemistry The Kumamoto Institute Of Technology

• Kobayashi Shinya

  National Research Institute Of Brewing

• SHIBATA HIROOMI

  Laboratory of Animal Environment, Meiji University

• YAMAGUCHI IKUKO

  Laboratory of Animal Environment, Meiji University

• SHIBATA HIROOMI

  Meiji University, Laboratory of Animal Environment

• TAKAHASHI MIKA

  Meiji University, Laboratory of Animal Environment

• YAMAGUCHI IKUKO

  Meiji University, Laboratory of Animal Environment

• KOBAYASHI SHIGEKI

  Meiji University, Laboratory of Animal Environment

• Shibata Haruka

  Laboratory Of Animal Environment Meiji University

• Yamaguchi Ikuko

  Laboratory Of Animal Environment Meiji University

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